Phenotypic and functional consequences of plt binding to monocytes and its association with clinical features in sle
Mariscal, Anaís (Institut d'Investigació Biomèdica Sant Pau)
Zamora, Carlos (Institut d'Investigació Biomèdica Sant Pau)
Magallares López, Berta Paula (Institut d'Investigació Biomèdica Sant Pau)
Salman-Monte, Tarek Carlos (Institut Hospital del Mar d'Investigacions Mèdiques)
Ortiz, M. Àngels (Institut d'Investigació Biomèdica Sant Pau)
Díaz Torne, César (Institut d'Investigació Biomèdica Sant Pau)
Castellvi, Ivan (Institut d'Investigació Biomèdica Sant Pau)
Corominas, Hèctor (Institut d'Investigació Biomèdica Sant Pau)
Vidal, Silvia (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2021
Abstract:	Platelets (PLTs) can modulate the immune system through the release of soluble mediators or through interaction with immune cells. Monocytes are the main immune cells that bind with PLTs, and this interaction is increased in several inflammatory and autoimmune conditions, including systemic lupus erythematosus (SLE). Our aim was to characterize the phenotypic and functional consequences of PLT binding to monocytes in healthy donors (HD) and in SLE and to relate it to the pathogenesis of SLE. We analyzed the phenotypic and functional features of monocytes with non-activated and activated bound PLTs by flow cytometry. We observed that monocytes with bound PLTs and especially those with activated PLTs have an up-regulated HLA-DR, CD86, CD54, CD16 and CD64 expression. Monocytes with bound PLTs also have an increased capacity for phagocytosis, though not for efferocytosis. In addition, monocytes with bound PLTs have increased IL-10, but not TNF-α, secretion. The altered phenotypic and functional features are comparable in SLE and HD monocytes and in bound PLTs. However, the percentages of monocytes with bound PLTs are significantly higher in SLE patients and are associated with undetectable levels of anti-dsDNA antibodies and hematuria, and with normal C3 and albumin/creatinine levels. Our results suggest that PLTs have a modulatory influence on monocytes and that this effect may be highlighted by an increased binding of PLTs to monocytes in autoimmune conditions.
Grants:	Instituto de Salud Carlos III PI17/00072 Instituto de Salud Carlos III PI20/00184
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Platelets ; Monocytes ; Lupus ; Immune modulation
Published in:	International journal of molecular sciences, Vol. 22 Núm. 9 (january 2021) , p. 4719, ISSN 1422-0067

DOI: 10.3390/ijms22094719
PMID: 33947017

17 p, 3.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles