CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
Pannunzio, Bruno (Universidad de la República. Departamento de Histología y Embriología)
Amo Aparicio, Jesús (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Julián, Camila (Universidad de la República. Departamento de Histología y Embriología)
López Vales, Rubén (Universitat Autònoma de Barcelona. Institut de Neurociències)
Peluffo, Hugo (Universitat de Barcelona. Departament de Biomedicina)
Lago, Natalia (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Universitat Autònoma de Barcelona. Institut de Neurociències

Date:	2022
Abstract:	Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.
Grants:	Agencia Estatal de Investigación PID2020-120267RB-I00 Instituto de Salud Carlos III R21/0017/008 European Commission 101030280
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; Versió publicada
Subject:	CD200R1 blocking antibody ; Immune receptor ; Macrophages ; Regeneration ; Functional reinnervation ; Wallerian Degeneration
Published in:	Cells, Vol. 11 (2022) , art. 1786, ISSN 2073-4409

DOI: 10.3390/cells11111786
PMID: 35681481

16 p, 2.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Published articles