How copper can impact pig growth : comparing the effect of copper sulfate and monovalent copper oxide on oxidative status, inflammation, gene abundance, and microbial modulation as potential mechanisms of action
Forouzandeh, Asal (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Blavi Josa, Laia (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Pérez Hernández, José Francisco (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
D'angelo, Matilde (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
González Solé, Francesc (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Monteiro, Alessandra (Animine)
Stein, Hans H (University of Illinois. Division of Nutritional Sciences)
Solà Oriol, David (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2022
Resum:	The beneficial effect of elevated concentrations of copper (Cu) on growth performance of pigs has been already demonstrated; however, their mechanism of action is not fully discovered. The objective of the present experiment was to investigate the effects of including Cu from copper sulfate (CuSO) or monovalent copper oxide (CuO) in the diet of growing pigs on oxidative stress, inflammation, gene abundance, and microbial modulation. We used 120 pigs with initial body weight (BW) of 11. 5 ± 0. 98 kg in 2 blocks of 60 pigs, 3 dietary treatments, 5 pigs per pen, and 4 replicate pens per treatment within each block for a total of 8 pens per treatment. Dietary treatments included the negative control (NC) diet containing 20 mg Cu/kg and 2 diets in which 250 mg Cu/kg from CuSO or CuO was added to the NC. On day 28, serum samples were collected from one pig per pen and this pig was then euthanized to obtain liver samples for the analysis of oxidative stress markers (Cu/Zn superoxide dismutase, glutathione peroxidase, and malondialdehyde, MDA). Serum samples were analyzed for cytokines. Jejunum tissue and colon content were collected and used for transcriptomic analyses and microbial characterization, respectively. Results indicated that there were greater (P < 0. 05) MDA levels in the liver of pigs fed the diet with 250 mg/kg CuSO than in pigs fed the other diets. The serum concentration of tumor necrosis factor-alpha was greater (P < 0. 05) in pigs fed diets containing CuSO compared with pigs fed the NC diet or the diet with 250 mg Cu/kg from CuO. Pigs fed diets containing CuSO or CuO had a greater (P < 0. 05) abundance of genes related to the intestinal barrier function and nutrient transport, but a lower (P < 0. 05) abundance of pro-inflammatory genes compared with pigs fed the NC diet. Supplementing diets with CuSO or CuO also increased (P < 0. 05) the abundance of Lachnospiraceae and Peptostreptococcaceae families and reduced (P < 0. 05) the abundance of the Rikenellaceae family, Campylobacter, and Streptococcus genera in the colon of pigs. In conclusion, adding 250 mg/kg of Cu from CuSO or CuO regulates genes abundance in charge of the immune system and growth, and promotes changes in the intestinal microbiota; however, CuO induces less systemic oxidation and inflammation compared with CuSO. Elevated concentrations of copper promote pig growth performance by modulating cytokines and intestinal microbes.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2019FI_B 00282
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Copper sulfate ; Gene abundance ; Monovalent copper oxide ; Microbiota ; Oxidative status ; Swine
Publicat a:	Journal of animal science, Vol. 100 (june 2022) , ISSN 1525-3163

DOI: 10.1093/jas/skac224
PMID: 35723874

12 p, 12.2 MB

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