Antimicrobial Resistant Salmonella in Chelonians : Assessing Its Potential Risk in Zoological Institutions in Spain
Marín Orenga, Clara (Grupo Estudio de la Medicina y Conservación de la Fauna Silvestre)
Martín-Maldonado, Bárbara (Universidad Europea de Madrid. Deparment of Veterinary Medicine)
Cerdà-Cuéllar, Marta (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Sevilla-Navarro, Sandra (Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana (CECAV), 12539 Les Alqueries, Spain)
Lorenzo-Rebenaque, Laura (Universidad Cardenal Herrera-CEU. Instituto de Ciencias Biomédicas)
Montoro-Dasi, Laura (Universidad Cardenal Herrera-CEU. Instituto de Ciencias Biomédicas)
Manzanares, Alicia (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Ayats, Teresa (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Mencía-Gutiérrez, Aida (Grupo de Rehabilitación de la Fauna Autóctona y su Hábitat)
Jordá, Jaume (Universidad Cardenal Herrera-CEU. Instituto de Ciencias Biomédicas)
González, Fernando (Grupo de Rehabilitación de la Fauna Autóctona y su Hábitat)
Rojo-Solís, Carlos (Avanqua Oceanogàfic. Oceanogràfic Veterinary Services)
Barros García, Carlos (Avanqua Oceanogàfic. Oceanogràfic Veterinary Services)
García-Párraga, Daniel (Avanqua Oceanogàfic. Oceanogràfic Veterinary Services)
Vega, Santiago (Grupo Estudio de la Medicina y Conservación de la Fauna Silvestre)

Date:	2022
Abstract:	Salmonella is mostly noted as a food-borne pathogen, but contact with chelonians has also been reported as a source of infection. Moreover, high levels of antimicrobial resistance (AMR) have been reported in Salmonella isolated from wild and captive reptiles. The aim of this study was to assess the occurrence of Salmonella AMR carriage by chelonians admitted to two zoological institutions in Spain, characterizing the isolates to assess the Salmonella AMR epidemiology in wildlife. To this end, 152 chelonians from nine species were sampled upon their arrival at the zoological nuclei. Salmonella identification was based on ISO 6579-1:2017 (Annex D), isolates were serotyped and their AMR analysed according to the EU Decision 2013/652. Moreover, the genetic relationship of the isolates was assessed by pulsed-field gel electrophoresis (PFGE). Results showed 19% (29/152) of the chelonians positive to Salmonella, all of them tortoises. For all isolates, 69% (20/29) were resistant and 34% (10/29) multidrug-resistant (MDR) strains. PFGE clustered isolates according to the serovar, confirming a low genetic diversity. In conclusion, this study shows a high presence of MDR Salmonella strains in tortoises at their entry into zoological nuclei. This condition highlights the need to establish Salmonella detection protocols for the entry of animals into these centres.
Grants:	Ministerio de Ciencia e Innovación PRE2019-087435
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Salmonella ; Antibiotic resistance ; Zoonoses ; Tortoises ; Wildlife
Published in:	Veterinary Sciences, Vol. 9 (may 2022) , ISSN 2306-7381

DOI: 10.3390/vetsci9060264
PMID: 35737316

12 p, 840.6 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles