Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
Gutiérrez-Martín, Daniel (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Marquès, Montse (Institut d'Investigació Sanitària Pere Virgili)
Pons-Escoda, Albert (Institut d'Investigació Biomèdica de Bellvitge)
Vidal, Noemi (Institut d'Investigació Biomèdica de Bellvitge)
Bruna, Jordi (Universitat Autònoma de Barcelona. Institut de Neurociències)
Restrepo-Montes, Esteban (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
López-Serna, Rebeca (Institute of Sustainable Processes (Valladolid))
García-Sayago, Francisco (Institut de Medicina Legal de Catalunya i Ciències Forenses (Lleida))
Majos, Carles (Institut d'Investigació Biomèdica de Bellvitge)
Gago-Ferrero, Pablo (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Gil-Solsona, Rubén (Institut de Diagnosi Ambiental i Estudis de l'Aigua)

Date:	2023
Abstract:	Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC-HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e. g. , pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i. e. , linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e. g. , R% within 60-120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
Grants:	Instituto de Salud Carlos III PI20/00360
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Human tissue ; Non-target screening ; Suspect screening ; Bead beating ; LC-HRMS ; Human biomonitoring ; Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
Published in:	MethodsX, Vol. 10 (february 2023) , ISSN 2215-0161

DOI: 10.1016/j.mex.2023.102069
PMID: 36879761

7 p, 523.1 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles