Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
Perlas Puente, Albert (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Bertran, Kateri (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Abad, Francesc X (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Borrego, Carles M. (Universitat de Girona. Institut d'Ecologia Aquàtica)
Nofrarías Espadamala, Miquel (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Valle, Rosa (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Pailler-García, Lola (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Ramis Salva, Antonio José (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Cortey, Martí (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Acuña, Vicenç (Universitat de Girona)
Majó i Masferrer, Natàlia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Date:	2023
Abstract:	Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i. e. , 10-13 °C vs. 16-18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e. g. , weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.
Grants:	Agencia Estatal de Investigación PID2020-114060RR-C33 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-BP-00105 Ministerio de Economía y Competitividad RyC-2015-17154
Note:	Altres ajuts: acords transformatius de la UAB
Note:	Instituto Nacional de Investigación y Tecnologı́a Agraria y Alimentaria PID2020-114060RR-C33-INFLUOMA
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Influenza ; Birds ; Water ; Environment ; Mediterranean ; Sediments
Published in:	Science of the total environment, Vol. 863 (october 2023) , p. 160902, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2022.160902
PMID: 36526195

9 p, 1.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles