Treatment with Hydrogen-Rich Water Improves the Nociceptive and Anxio-Depressive-like Behaviors Associated with Chronic Inflammatory Pain in Mice
Coral-Pérez, Santiago (Institut d'Investigació Biomèdica Sant Pau)
Martínez-Martel, Ignacio (Institut d'Investigació Biomèdica Sant Pau)
Martínez-Serrat, Maria (Institut d'Investigació Biomèdica Sant Pau)
Batallé Melgarejo, Gerard (Institut d'Investigació Biomèdica Sant Pau)
Bai, Xue (Institut d'Investigació Biomèdica Sant Pau)
Leite-Panissi, C.R.A. (Department of Psychology. Faculty of Philosophy Science and Letters of Ribeirão Preto. University of São Paulo)
Pol, Olga (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2022
Abstract:	Chronic inflammatory pain is manifested in many diseases. The potential use of molecular hydrogen (H) as a new therapy for neurological disorders has been demonstrated. Recent studies prove its analgesic properties in animals with neuropathic pain, but the possible antinociceptive, antidepressant, and/or anxiolytic actions of H during persistent inflammatory pain have not been investigated. Therefore, using male mice with chronic inflammatory pain incited by the subplantar injection of complete Freud's adjuvant (CFA), we assessed the actions of hydrogen-rich water (HRW) systemically administered on: (1) the nociceptive responses and affective disorders associated and (2) the oxidative (4-hydroxy-2-nonenal; 4-HNE), inflammatory (phosphorylated-NF-kB inhibitor alpha; p-IKBα), and apoptotic (Bcl-2-like protein 4; BAX) changes provoked by CFA in the paws and amygdala. The role of the antioxidant system in the analgesia induced by HRW systemically and locally administered was also determined. Our results revealed that the intraperitoneal administration of HRW, besides reducing inflammatory pain, also inhibited the depressive- and anxiolytic-like behaviors associated and the over expression of 4-HNE, p-IKBα, and BAX in paws and amygdala. The contribution of the nuclear factor erythroid 2-related factor 2/heme oxygenase 1 and NAD(P)H: quinone oxidoreductase 1 pathway in the analgesic activities of HRW, systemically or locally administered, was also shown. These data revealed the analgesic, antidepressant, and anxiolytic actions of HRW. The protective, anti-inflammatory, and antioxidant qualities of this treatment during inflammatory pain were also demonstrated. Therefore, this study proposes the usage of HRW as a potential therapy for chronic inflammatory pain and linked comorbidities.
Grants:	Instituto de Salud Carlos III PI18/00645 Instituto de Salud Carlos III PI21/0059
Note:	Altres ajuts: Ministerio de Ciencia, Innovación y Universidades; Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea; CERCA Programme/Generalitat de Catalunya; Conselho Nacional de Desenvolvimento Científico e Tecnológico-Brazil (CNPq 309215/2019-6).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Allodynia ; Anxiety ; Apoptosis ; Depression ; Hyperalgesia ; Inflammatory pain ; Molecular hydrogen ; Oxidative stress
Published in:	Antioxidants, Vol. 11 Núm. 11 (november 2022) , p. 2153, ISSN 2076-3921

DOI: 10.3390/antiox11112153
PMID: 36358525

16 p, 2.1 MB

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