GC-MS/MS Determination of Synthetic Cathinones : 4-chloromethcathinone, N-ethyl Pentedrone, and N-ethyl Hexedrone in Oral Fluid and Sweat of Consumers under Controlled Administration: Pilot Study
Nuñez-Montero, Melani (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lombroni, Claudia (Univesità degli Studi di Torino)
Maida, Nunzia La (Istituto Superiore di Sanità)
Rotolo, Maria Concetta (Istituto Superiore di Sanità)
Pichini, Simona (Istituto Superiore di Sanità)
Papaseit, Esther (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Hladun, Olga (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ventura, Mireia (Associació Benestar i Desenvolupament)
Poyatos, Lourdes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pérez-Mañá, Clara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Farre, Magi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Marchei, Emilia (Istituto Superiore di Sanità)
Universitat Autònoma de Barcelona

Date:	2023
Abstract:	This study presents a validated GC-MS/MS method for the detection and quantification of 4-chloromethcathinone or clephedrone (4-CMC), N-ethyl Pentedrone (NEP), and N-ethyl Hexedrone (NEH, also named HEXEN) in oral fluid and sweat and verifies its feasibility in determining human oral fluid concentrations and pharmacokinetics following the administration of 100 mg of 4-CMC orally and 30 mg of NEP and NEH intranasally. A total of 48 oral fluid and 12 sweat samples were collected from six consumers. After the addition of 5 μL of methylone-d and 200 μL of 0. 5 M ammonium hydrogen carbonate, an L/L extraction was carried out using ethyl acetate. The samples, dried under a nitrogen flow, were then derivatized with pentafluoropropionic anhydride and dried again. One microliter of the sample reconstituted in 50 μL of ethyl acetate was injected into GC-MS/MS. The method was fully validated according to international guidelines. Our results showed how, in oral fluid, the two cathinones taken intranasally were absorbed very rapidly, within the first hour, when compared with the 4-CMC which reached its maximum concentration peak in the first three hours. We observed that these cathinones were excreted in sweat in an amount equivalent to approximately 0. 3% of the administered dose for 4-CMC and NEP. The total NEH excreted in sweat 4 h after administration was approximately 0. 2% of the administered dose. Our results provide, for the first time, preliminary information about the disposition of these synthetic cathinones in the consumers' oral fluid and sweat after controlled administration.
Grants:	Instituto de Salud Carlos III PI20/00879 Instituto de Salud Carlos III PI17/01962
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Gas-chromatography tandem mass spectrometry ; N-ethyl pentedrone ; N-ethyl hexedrone ; Oral fluid pharmacokinetics ; Sweat ; Synthetic cathinones ; 4-chloromethcathinone
Published in:	International journal of molecular sciences, Vol. 24 (may 2023) , ISSN 1422-0067

DOI: 10.3390/ijms24119387
PMID: 37298339

15 p, 1.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles