Faecal phageome of healthy individuals : Presence of antibiotic resistance genes and variations caused by ciprofloxacin treatment
Fernandez-Orth, Dietmar (Barcelona Institute for Global Health (ISGlobal))
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
Brown-Jaque, Maryury (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Rodríguez-Rubio, Lorena (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Espinal, Paula (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Rodríguez-Navarro, Judith (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron)
Muniesa, Maite (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)

Data:	2019
Descripció:	11 pàg.
Resum:	Objectives: Antimicrobial resistance genes (ARGs) can be transferred by means of mobile genetic elements, which play a critical role in the dissemination of resistance in the bacterial community. ARG transmission within mobile genetic elements has been reported in plasmids and transposons but less frequently in bacteriophages. Here, the bacteriophage fraction of seven human faecal samples was purified and deep-sequenced to detect the presence of ARGs in the phage particles. Methods: Seven faecal samples (five from healthy individuals and two from a patient before and after receiving ciprofloxacin treatment) were used to extract phage DNA, which was purified and then sequenced in a MiSeq (Illumina). Generated reads were checked for quality and assembled, and then the generated contigs analysed with Kraken, PHASTER, VirSorter and Prokka. Some genes were also validated by quantitative PCR. Results: Analysis of the purified phage DNA by Kraken identified from 4 to 266 viruses in the samples. The viral fraction corresponded mainly to the order Caudovirales, including phages from the Siphoviridae and Myoviridae families. Bacterial genes associated with antimicrobial resistance were detected in the viral DNA, as confirmed by quantitative PCR. Higher densities of ARG-carrying phage particles were observed in the post- versus preciprofloxacin treatment sample. Conclusions: The finding of ARGs in phage particles supports the description of phages as mobile elements contributing to the dissemination of bacterial antibiotic resistance and suggests ciprofloxacin treatment may play a role in the release of ARG-carrying particles, thereby increasing resistance.
Ajuts:	Ministerio de Economía y Competitividad RD12/0015/0017 Ministerio de Economía y Competitividad PI13/00329 Ministerio de Economía y Competitividad CD15/00017
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	SDG 3 - Good Health and Well-being
Publicat a:	Journal of antimicrobial chemotherapy, Vol. 74, Issue 4 (April 2019) , p. 854-864, ISSN 1460-2091

DOI: 10.1093/jac/dky540
PMID: 30649322

Postprint 35 p, 396.5 KB

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