The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization
Kashyap, Anurag (Centre de Recerca en Agrigenòmica)
Jiménez-Jiménez, Álvaro (Centre de Recerca en Agrigenòmica)
Figueras, Mercè (Universitat de Girona. Departament de Biologia)
Serra, Olga (Universitat de Girona. Departament de Biologia)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Data:	2023
Resum:	As a wall polymer, suberin has a multifaceted role in plant development and stress responses. It is deposited between the plasma membrane and the primary cell wall in specialized tissues such as root exodermis, endodermis, phellem, and seed coats. It is formed de novo in response to stresses such as wounding, salt injury, drought, and pathogen attack and is a complex polyester mainly consisting of fatty acids, glycerol, and minor amounts of ferulic acid that are associated to a lignin-like polymer predominantly composed of ferulates. Metabolomic and transcriptomic studies have revealed that cell wall lignification precedes suberin deposition. The ferulic acid esterified to ω-hydroxy fatty acids, synthetized by the feruloyl transferase FHT (or ASFT), presumably plays a role in coupling both polymers, although the precise mechanism is not understood. Here, we use the promoter of tomato suberin feruloyl transferase (FHT/ASFT) fused to GUS (β-glucuronidase) to demonstrate that ferulate deposition agrees with the site of promoter FHT activation by using a combination of histochemical staining and UV microscopy. Hence, FHT promoter activation and alkali UV microscopy can be used to identify the precise localization of early suberizing cells rich in ferulic acid and can additionally be used as an efficient marker of early suberization events during plant development and stress responses. This line can be used in the future as a tool to identify emerging suberization sites via ferulate deposition in tomato plants, which may contribute to germplasm screening in varietal improvement programs.
Ajuts:	Agencia Estatal de Investigación PID2019-108595RB-I00 Agencia Estatal de Investigación TED2021-131457B-I00 Agencia Estatal de Investigación CEX2019-000917
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Suberin ; Ferulates ; Stress ; Development ; Tomato
Publicat a:	Plants, Vol. 12, Issue 9 (May 2023) , art. 1890, ISSN 2223-7747

DOI: 10.3390/plants12091890
PMID: 37176949

14 p, 3.9 MB

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