Susceptibility Evaluation to Fire Blight and Genome-Wide Associations within a Collection of Asturian Apple Accessions
García-Fernández, Belén (Regional Service for Agrofood Research and Development)
Dolcet-Sanjuan, Ramon (IRTA Fruitcentre)
Micheletti, Diego (Research and Innovation Center)
Antón-Díaz, María José (Regional Service for Agrofood Research and Development)
Solsona, Cristina (IRTA Fruitcentre)
Fernández Ramos, Mercedes (Regional Service for Agrofood Research and Development)
Abad Morejón de Girón, Xavier (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Dapena, Enrique (Regional Service for Agrofood Research and Development)

Date:	2023
Abstract:	Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom ® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches.
Grants:	Ministerio de Ciencia e Innovación PRE2018-086417 Ministerio de Ciencia, Innovación y Universidades RTA2017-00102-C03-01 Ministerio de Ciencia, Innovación y Universidades RTA2017-00102-C03-02 European Commission. Horizon 2020 101061028
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Malus domestica ; Erwinia amylovora ; Cider apple ; Apple bank germplasm ; Phenotypic evaluation ; Local cultivars ; Artificial inoculations ; Genome-wide association studies ; Apple breeding ; Putative candidate genes
Published in:	Plants, Vol. 12 (december 2023) , ISSN 2223-7747

DOI: 10.3390/plants12234068
PMID: 38068703

13 p, 2.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles