Properties and cellular uptake of photo-triggered mixed metallosurfactant vesicles intended for controlled CO delivery in gas therapy
Trallero, Jan (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Camacho, Mercedes (Institut d'Investigació Biomèdica Sant Pau)
Marín García, Maribel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Alvarez Marimon, Maria Elena (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Benseny Cases, Núria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Barnadas Rodríguez, Ramon (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2023
Description:	11 pàg.
Abstract:	The scientific relevance of carbon monoxide has increased since it was discovered that it is a gasotransmitter involved in several biological processes. This fact stimulated research to find a secure and targeted delivery and lead to the synthesis of CO-releasing molecules. In this paper we present a vesicular CO delivery system triggered by light composed of a synthetized metallosurfactant (TCOL10) with two long carbon chains and a molybdenum-carbonyl complex. We studied the characteristics of mixed TCOL10/phosphatidylcholine metallosomes of different sizes. Vesicles from 80 to 800 nm in diameter are mainly unilamellar, do not disaggregate upon dilution, in the dark are physically and chemically stable at 4 °C for at least one month, and exhibit a lag phase of about 4 days before they show a spontaneous CO release at 37 °C. Internalization of metallosomes by cells was studied as function of the incubation time, and vesicle concentration and size. Results show that large vesicles are more efficiently internalized than the smaller ones in terms of the percentage of cells that show TCOL10 and the amount of drug that they take up. On balance, TCOL10 metallosomes constitute a promising and viable approach for efficient delivery of CO to biological systems.
Grants:	Agencia Estatal de Investigación PID2021-123682OB-I00
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	CORM ; Cell internalization ; Colloid ; Liposome ; Metallosome ; Self-assembly
Published in:	Colloids and surfaces. Biointerfaces, Vol. 228 (2023) , p. 113422, ISSN 1873-4367

DOI: 10.1016/j.colsurfb.2023.113422
PMID: 37356136

11 p, 4.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles