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| Home > Articles > Published articles > Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease |
(Institut d'Investigacions Biomèdiques August Pi i Sunyer) (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras) (Department of Molecular Life Sciences. University of Zurich. Switzerland. SIB Swiss Institute of Bioinformatics) (Centro de Investigaciones Biológicas. Consejo Superior de Investigaciones Científicas (CSIC)) (NanoString Technologies) (NanoString Technologies) (Centre de Regulació Genòmica) (Centre de Regulació Genòmica) (Hospital Universitari MútuaTerrassa (Terrassa, Catalunya)) (Hospital Universitari MútuaTerrassa (Terrassa, Catalunya)) (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras) (Universitat Pompeu Fabra)| Date: | 2023 |
| Abstract: | Ulcerative colitis and Crohn's disease are chronic inflammatory intestinal diseases with perplexing heterogeneity in disease manifestation and response to treatment. While the molecular basis for this heterogeneity remains uncharacterized, single-cell technologies allow us to explore the transcriptional states within tissues at an unprecedented resolution which could further understanding of these complex diseases. Here, we apply single-cell RNA-sequencing to human inflamed intestine and show that the largest differences among patients are present within the myeloid compartment including macrophages and neutrophils. Using spatial transcriptomics in human tissue at single-cell resolution (CosMx Spatial Molecular Imaging) we spatially localize each of the macrophage and neutrophil subsets identified by single-cell RNA-sequencing and unravel further macrophage diversity based on their tissue localization. Finally, single-cell RNA-sequencing combined with single-cell spatial analysis reveals a strong communication network involving macrophages and inflammatory fibroblasts. Our data sheds light on the cellular complexity of these diseases and points towards the myeloid and stromal compartments as important cellular subsets for understanding patient-to-patient heterogeneity. |
| Grants: | European Commission. Horizon 2020 848028 European Commission. Horizon 2020 831434 Agencia Estatal de Investigación PID2020-115439GB-I00 Agencia Estatal de Investigación RTI2018-096946-B-I00 |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Subject: | Crohn Disease ; Humans ; Inflammatory Bowel Diseases ; Macrophages ; Neutrophils ; RNA |
| Published in: | Nature communications, Vol. 14 Núm. 1 (december 2023) , p. 4506, ISSN 2041-1723 |
