Keystone bacterial functional module activates P-mineralizing genes to enhance enzymatic hydrolysis of organic P in a subtropical forest soil with 5-year N addition
Zeng, Quanxin (Fujian Normal University)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Sardans i Galobart, Jordi (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Zhang, Qiufang (Fujian Normal University)
Zhou, Jiacong (Fujian Normal University)
Yue, Kai (Fujian Normal University)
Chen, Yuehmin (Fujian Normal University. Institute of Geography)
Yang, Yusheng (Fujian Normal University. Institute of Geography)
Fan, Yuexin (Fujian Normal University. Institute of Geography)

Data:	2024
Resum:	Microorganisms play an integral role in driving phosphorus (P) transformation in forest soils; however, studies on soil P cycling and the molecular mechanisms of microbes activated in response to elevated nitrogen (N) deposition are limited. In this study, we conducted a multilevel field N enrichment experiment in a subtropical P-deficient Moso bamboo (Phyllostachys heterocycla) system to evaluate the microbial ecological traits of P transformation (e. g. , organic P mineralization and inorganic P solubilization) over three consecutive years. N addition significantly decreased available and organic P levels in the soil and increased the microbial biomass C:P and N:P ratios, indicative of severe microbial P limitation. Consequently, N addition increased the absolute abundance of P starvation response regulation genes (phoU and phoR), which further induced an increase in organic P mineralization (phoN, phoD, appA), but not that of inorganic P solubilization genes (ppx and gcd). This suggests that microbes enhance P availability by organic P mineralization rather than inorganic P solubilization to ameliorate reduced P availability. Furthermore, a bacterial functional module (B_Mod#0) consisting of Proteobacteria, Actinobacteria, and Firmicutes accounted for more than 60% of the changes in the abundance of genes responsible for organic P-mineralization in the soil, suggesting that B_Mod#0 acts as a keystone phylotype in enhancing functional P-cycling potential. This study provides novel insights into microorganism-driven P cycling in P-deficient forest soils with N addition.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Nitrogen deposition ; Phosphorus limitation ; Microbial modules ; Functional P41 cycling potential ; Phosphorus mineralization
Publicat a:	Soil Biology and Biochemistry, Vol. 192 (May 2024) , art. 109383, ISSN 1879-3428

DOI: 10.1016/j.soilbio.2024.109383

Disponible a partir de: 2026-05-31 Postprint

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