Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease : A cross-platform validation study
Hok-A-Hin, Yanaika S. (Amsterdam UMC. University Medical Center)
Bolsewig, Katharina (VU University Medical Center)
Ruiters, Daimy N. (VU University Medical Center)
Lleó, Alberto (Institut d'Investigació Biomèdica Sant Pau)
Alcolea, Daniel (Institut d'Investigació Biomèdica Sant Pau)
Lemstra, Afina W. (Vrije Universiteit Amsterdam)
van der Flier, Wiesje M (VU University Medical Centers)
Teunissen, Charlotte E (VU University Medical Center)
del Campo, Marta (Fundació Pasqual Maragall)
Universitat Autònoma de Barcelona

Fecha:	2023
Resumen:	Our previous antibody-based cerebrospinal fluid (CSF) proteomics study showed that Thimet oligopeptidase (THOP1), an amyloid beta (Aβ) neuropeptidase, was increased in mild cognitive impairment with amyloid pathology (MCI-Aβ+) and Alzheimer's disease (AD) dementia compared with controls and dementia with Lewy bodies (DLB), highlighting the potential of CSF THOP1 as an early specific biomarker for AD. We aimed to develop THOP1 immunoassays for large-scale analysis and validate our proteomics findings in two independent cohorts. We developed in-house CSF THOP1 immunoassays on automated Ella and Simoa platforms. The performance of the different assays were compared using Passing-Bablok regression analysis in a subset of CSF samples from the discovery cohort (n = 72). Clinical validation was performed in two independent cohorts (cohort 1: n = 200; cohort 2: n = 165) using the Ella platform. THOP1 concentrations moderately correlated between proteomics analysis and our novel assays (Rho > 0. 580). In both validation cohorts, CSF THOP1 was increased in MCI-Aβ+ (>1. 3-fold) and AD (>1. 2-fold) compared with controls; and between MCI-Aβ+ and DLB (>1. 2-fold). Higher THOP1 concentrations were detected in AD compared with DLB only when both cohorts were analyzed together. In both cohorts, THOP1 correlated with CSF total tau (t-tau), phosphorylated tau (p-tau), and Aβ40 (Rho > 0. 540) but not Aβ42. Validation of our proteomics findings underpins the potential of CSF THOP1 as an early specific biomarker associated with AD pathology. The use of antibody-based platforms in both the discovery and validation phases facilitated the translation of proteomics findings, providing an additional workflow that may accelerate the development of biofluid-based biomarkers.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Alzheimer's disease ; Biomarkers ; CSF ; THOP1
Publicado en:	Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, Vol. 15 (july 2023) , ISSN 2352-8729

DOI: 10.1002/dad2.12456
PMID: 37502019

11 p, 2.0 MB

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