2025-09-30
12:07 |
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2025-09-29
20:40 |
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30 p, 1.1 MB |
Evaluación de la utilidad del test ex vivo de depósitos de C5b9 para monitorizar el estado del sistema complemento en pacientes con nefropatía lúpica, glomerulopatía membranosa y nefropatía por IgA
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Bustamante Echeverri, Catalina ;
Caixa Jacobs, Conxita, dir. ;
Ferrer Costa, Roser, dir. (Hospital Universitari Vall d'Hebron) ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
The complement system (CS) is a key component of innate immunity that culminates in the formation of the membrane attack complex (MAC or C5b9) on the membranes of pathogens or apoptotic cells, and its activation can occur through three pathways: the classical, the lectin, and the alternative pathways, where the first two are mainly triggered by the recognition of microorganisms or immune complexes, while the alternative pathway is constitutively activated through spontaneous hydrolysis of C3, and although this process is physiologically regulated by complement inhibitors, in certain kidney diseases such as atypical hemolytic uremic syndrome, this pathway can be overactivated, and overactivation of the CS has also been described in other complex glomerulopathies, although there are currently no reliable methods to assess its contribution in these diseases, so the objective of this study was to evaluate the utility of the ex vivo C5b9 deposits test on human endothelial cells (HMEC-1) as a tool to detect complement activation in complex glomerulopathies, and serum samples from patients with lupus nephritis (LN), membranous glomerulopathy (MG), and IgA nephropathy (IgAN) were analyzed, where in LN, C5b9 deposits were associated with higher lupus activity assessed by the SLEDAI index, and in MG, no clear correlation was found with clinical parameters although there was a slight association with renal function deterioration, while in IgAN, deposits were linked to greater histological damage according to the MEST-C score of the Oxford classification found in kidney biopsies, and although serum levels of C3 and C4 were measured, no consistent correlations were observed, suggesting these do not reliably reflect CS activity, so in conclusion, the ex vivo C5b-9 deposits test could represent a useful complementary diagnostic tool in the assessment of the complement system in complex glomerulopathies when traditional serum markers are inconclusive.
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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2025-09-29
20:06 |
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30 p, 1.4 MB |
Impact of lactate on exosomes and its role in acute pancreatitis-associated inflammation
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Canalda Batalla, Noemi ;
Closa Autet, Daniel 1961-, dir. (Institut d'Investigacions Biomèdiques de Barcelona) ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
Acute pancreatitis (AP) is an inflammatory condition often associated with systemic complications, and macrophages are key regulators of the inflammatory response, while exosomes, a subtype of small extracellular vesicles (sEVs), are important mediators of intercellular crosstalk and can influence immune cell behavior, and lactated Ringer's solution (LR), commonly used in AP treatment, contains lactate, which has demonstrated anti-inflammatory effects, so this study explores whether exosome exposure to lactate modifies their effect on macrophage inflammatory response. [...]
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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2025-09-29
16:50 |
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33 p, 1.2 MB |
Optimization of Enzyme Replacement Therapy Using Vesicles as Delivery Vehicles for alpha galactosidase (GLA) for the treatment of Fabry disease
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Coello Quihuiri, Edwin Patricio ;
Abasolo, Ibane, dir. (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
In the first part of the project, the production of α-galactosidase A (GLA) was carried out using two strategies: transient transfection in HEK293 cells and stable cloning in CHO cells, and three variants were obtained: HEK GLA HIS, HEK GLA cMYC, and CHO GLA CMYC, with the highest expression observed in CHO GLA CMYC, in line with previous reports highlighting CHO cells for their high efficiency and stability in recombinant protein production; the HEK GLA CMYC variant outperformed HEK GLA HIS, potentially due to the stabilizing effect of the CMYC tag, while in contrast, the HIS tag may negatively affect the solubility and functionality of GLA, and for purification, soluble GLA was isolated using affinity chromatography with His SpinTrap columns, although exposure to imidazole reduced enzymatic activity, necessitating a subsequent dialysis step; the second phase of the project focused on the purification of extracellular vesicles (EVs) loaded with GLA, using two methods: bottom-through filtration (BTF) and tangential flow filtration (TFF), where TFF outperformed BTF in terms of yield and preservation of enzymatic activity owing to its gentler flow conditions, and CHO GLA CMYC cells not only produced a higher quantity of EVs but also generated vesicles with enhanced enzymatic activity, which is attributed to the superior folding capacity and post-translational modification machinery of CHO cells, so the CHO GLA CMYC system combined with TFF purification emerges as an efficient and scalable platform for the development of an extracellular vesicle-based enzyme replacement therapy (ERT) for Fabry disease.
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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2025-09-29
14:44 |
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33 p, 1.3 MB |
De 2D a 3D: cómo la arquitectura tumoral modula la respuesta a temozolomida e inmunogenicidad en el modelo de glioblastoma GL261
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Crespo Hereu, Mercedes ;
Candiota Silveira, Ana Paula, dir. (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ;
Fernández Cabada, Tamara, dir. ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
Glioblastoma (GB) is the most aggressive primary brain tumor in adults, and its marked heterogeneity, diffuse infiltration, and a strongly immunosuppressive tumor microenvironment underlie the poor prognosis and near-inevitable relapses; temozolomide (TMZ) remains the backbone of standard therapy and can modulate antitumor immunity via immunogenic cell death (ICD), in which signals such as calreticulin (CRT) exposure and the release of ATP and High Mobility Group Box 1 (HMGB1) act as immune activators; in parallel, preclinical progress requires models that better recapitulate tumor physiology, and three-dimensional GL261 spheroids are proposed as an alternative to 2D monolayers, as they reproduce gradients, cell-cell contacts, and spatial heterogeneity, and this work contrasts the response to TMZ in 2D versus 3D, focusing on viability and immunogenicity; overall, the transition to 3D yields compact, viable spheroids and, considered together, 2D and 3D preparations show comparable susceptibility after longer exposures, with a slight tendency toward greater preservation of viability in 3D, attributable to gradients of drug penetration, oxygen, and nutrients; the ICD signals analyzed display modest, time-dependent changes: CRT exposure appears modest and transient, ATP tends to increase at early time points and decrease later, and HMGB1 may rise initially and decline as damage progresses; taken together, 3D confers biological value even with modest differences versus 2D and emerges as a useful platform for further optimization, and integrated evaluations (accounting for synergy among signals) with higher temporal resolution, along with the incorporation of additional immunogenic signals, could better capture relevant immunogenicity, moreover, preliminary internal observations suggest that implanted spheroids recapitulate in vivo features, reinforcing their translational potential.
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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2025-09-26
16:13 |
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32 p, 5.2 MB |
¿Los cambios en la función de las plaquetas contribuyen al desarrollo de la demencia con cuerpos de Lewy?
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Fernández Aixalà, Kilian ;
Canalias Reverter, Francesca, dir. (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ;
Beyer, Katrin, dir. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
Lewy body dementia (DLB) is a neurodegenerative disease characterized by a combination of cognitive, motor, and neuropsychiatric symptoms, which complicates its differential diagnosis compared to other pathologies such as Alzheimer's disease (AD) and Parkinson's disease (PD). [...]
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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2025-09-26
15:32 |
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2025-09-26
15:16 |
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2025-09-26
14:56 |
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2025-09-25
18:35 |
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32 p, 1.7 MB |
Synaptic dysfunction induced by Aβ oligomers in Alzheimer's disease: AKAP150-NFAT signalling as a molecular target
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Román, Elena Ortiz de Zárate ;
Rodríguez Álvarez, José, dir. ;
Miñano Molina, Alfredo Jesús (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ;
Universitat Autònoma de Barcelona.
Facultat de Biociències
Alzheimer's disease (AD) is the most common form of dementia worldwide and to this day no effective cure has been found. Various studies suggest that synaptic dysfunction is one of the earliest pathological events in this disease, preceding even clinical symptoms. [...]
signalling pathway, which includes scaffolding protein AKAP79/150, phosphatase calcineurin (CaN) and transcription factor NFATc3. In this research, we observed that.
Aβo reduce the expression of AKAP150 in a time-dependent manner. This reduction was not prevented by the blocking of L-type calcium channels (LTCCs), suggesting that LTCCs are not involved in Aβo-induced AKAP150 decrease. [...]
contribute to synaptic dysfunction in AD and open the possibility of identifying these compounds as therapeutical targets.
2025
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599]
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