'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure
Ramió-Lluch, Laura (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Peña, Alejandro (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Colás, C. (Univerisdad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular)
Cebrián-Pérez, José A.. (Univerisdad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular)
Muiño-Blanco, T. (Univerisdad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular)
Ramírez, A. (Austral University of Chile. Biochemistry Institute and Animal Reproduction Institute)
Concha, Ilona I (Austral University of Chile. Biochemistry Institute and Animal Reproduction Institute)
Rigau, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2010
Resum:	The main scope of this manuscript is to analyse the dynamics of mitochondrial activity in boar sperm subjected to 'in vitro' capacitation (IVC) and subsequent progesterone-induced 'in vitro' acrosome reaction (IVAR). This was determined after analysis of the rhythm of O(2) consumption and concomitant changes in the mitochondria activity-specific JC-1 staining. Results showed that IVC, and especially IVAR, was concomitant with a peak in O(2) consumption (from 1. 61 ± 0. 08 nmol O(2)/min/10(7) viable sperm at 0 h of incubation to 2. 62 ± 0. 12 nmol O(2) /min/10(7) viable sperm after 5 min of IVAR induction). These results were accompanied by parallel changes in the mean intensity of JC-1 staining. Based on JC-1, mitochondrial activation followed a nucleated pattern, with specific, activation starting points at the midpiece from which mitochondrial activation was spread. Moreover, four separate sperm subpopulations were detected following the JC-1 orange-red/green ratio, and the observed changes in the mean JC-1 staining during IVC and IVAR were related to concomitant changes in both the orange-red/green JC-1 ratio and the percentage of sperm included in each subpopulation. All of these results indicate that IVC and the first minutes of IVAR are accompanied by a progressive increase in mitochondrial activity, which reached a peak coincidental with the achievement of IVAR. Moreover, results suggest the presence of separate sperm subpopulations, which show a different mitochondrial sensitivity to IVC and IVAR. Finally, mitochondrial activation, at least under JC-1 staining, seems to originate in concrete nucleation points at the midpiece, thus suggesting thus a well-coordinated pattern in boar-sperm mitochondrial activity modulation.
Ajuts:	Ministerio de Ciencia e Innovación AGL2004-04756-C02/02/GAN Ministerio de Ciencia e Innovación AGL2008-01792GAN
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Català
Document:	Article ; recerca ; Versió publicada
Matèria:	Senglars
Publicat a:	Reproduction in domestic animals (1990), Vol. 45 (2010) , p. 81-81, ISSN 0936-6768

DOI: 10.1111/j.1439-0531.2010.01655.x
PMID: 21121968

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