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Validation of the long-term assessment of hypothalamic-pituitary-adrenal activity in rats using hair corticosterone as a biomarker
Scorrano Fabrizio (Università degli studi (Bolonya, Itàlia). Unità di Fisiologia Animale)
Carrasco Trancoso, Javier (Universitat Autònoma de Barcelona. Unitat de Fisiologia animal)
Pastor Ciurana, Jordi (Universitat Autònoma de Barcelona. Unitat de Fisiologia animal)
Belda Poyato, Francisco Javier (Universitat Autònoma de Barcelona. Unitat de Fisiologia animal)
Rami Bastante, Alicia (Universitat Autònoma de Barcelona. Unitat de Fisiologia animal)
Bacci, Maria Laura (Università degli studi (Bolonya, Itàlia). Unità di Fisiologia Animale)
Armario García, Antonio (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date: 2015
Abstract: The evaluation of chronic activity of the hypothalamic-pituitary-adrenal (HPA) axis is critical for determining the impact of chronic stressful situations. However, current methods have important limitations. The potential use of hair glucocorticoids as a noninvasive retrospective biomarker of long-term HPA activity is gaining acceptance in humans and wild animals. However, there is no study examining hair corticosterone (HC) in laboratory animals. The present study validates a method for measuring HC in rats and demonstrates that it properly reflects chronic HPA activity. The HC concentration was similar in male and female rats, despite higher total plasma corticosterone levels in females, tentatively suggesting that it reflects free rather than total plasma corticosterone. Exposure of male rats to 2 different chronic stress protocols (chronic immobilization and chronic unpredictable stress) resulted in similarly higher HC levels compared to controls (1. 8-fold). HC also increased after a mild chronic stressor (30 min daily restraint). Chronic administration of 2 different doses of a long-acting ACTH preparation dramatically increased HC (3. 1- and 21. 5-fold, respectively), demonstrating that a ceiling effect in HC accumulation is unlikely under other more natural conditions. Finally, adrenalectomy significantly reduced HC. In conclusion, HC measurement in rats appears appropriate to evaluate integrated chronic changes in circulating corticosterone.
Rights: Tots els drets reservats
Language: Anglès.
Document: article ; recerca ; submittedVersion
Subject: ACTH ; Chronic immobilization ; Chronic unpredictable stress ; Glucocorticoids
Published in: FASEB Journal, Vol. 29 No. 3 (March 2015) , ISSN 0892-6638

DOI: 10.1096/fj.14-254474


Pre-print
31 p, 321.6 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles

 Record created 2014-12-04, last modified 2019-02-05



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