Web of Science: 26 cites, Scopus: 28 cites, Google Scholar: cites
Production of functional inclusion bodies in endotoxin-free Escherichia coli
Rueda, Fabián (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Mamat, Uwe (Forschungszentrum Borstel)
Wilke, Kathleen (Forschungszentrum Borstel)
Seras-Franzoso, Joaquín (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
García Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data: 2014
Resum: Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells.
Nota: Número d'acord de subvenció MINECO/RTA2012-00028-C02-02
Nota: Número d'acord de subvenció AGAUR/2014/SGR-132
Drets: Tots els drets reservats.
Llengua: Anglès.
Document: article ; recerca ; acceptedVersion
Matèria: E. coli ; Recombinant protein ; Inclusion bodies ; Endotoxin ; LPS ; LPS-free
Publicat a: Applied microbiology and biotechnology, Vol. 98 issue 22 (Nov. 2014) , p. 9229-9238, ISSN 0175-7598

DOI: 10.1007/s00253-014-6008-9

18 p, 391.8 KB

8 p, 520.1 KB

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