Web of Science: 17 citations, Scopus: 16 citations, Google Scholar: citations,
Functional Metagenomics of the Bronchial Microbiome in COPD
Millares Costas, Laura (Institut Germans Trias i Pujol)
Pérez-Brocal Vicente (Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO))
Ferrari, Rafaela (Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO))
Gallego, Miguel (Corporació Sanitària Parc Taulí)
Pomares Amigo, Xavier (Corporació Sanitària Parc Taulí)
García Núñez, Marian (Institut Germans Trias i Pujol)
Montón Concepción (Corporació Sanitària Parc Taulí)
Capilla Rubio, Sílvia (Corporació Sanitària Parc Taulí)
Monsó Molas, Eduard (Corporació Sanitària Parc Taulí)
Moya, Andrés (Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO))
Universitat Autònoma de Barcelona

Date: 2015
Abstract: The course of chronic obstructive pulmonary disease (COPD) is frequently aggravated by exacerbations, and changes in the composition and activity of the microbiome may be implicated in their appearance. The aim of this study was to analyse the composition and the gene content of the microbial community in bronchial secretions of COPD patients in both stability and exacerbation. Taxonomic data were obtained by 16S rRNA gene amplification and pyrosequencing, and metabolic information through shotgun metagenomics, using the Metagenomics RAST server (MG-RAST), and the PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) programme, which predict metagenomes from 16S data. Eight severe COPD patients provided good quality sputum samples, and no significant differences in the relative abundance of any phyla and genera were found between stability and exacerbation. Bacterial biodiversity (Chao1 and Shannon indexes) did not show statistical differences and beta-diversity analysis (Bray-Curtis dissimilarity index) showed a similar microbial composition in the two clinical situations. Four functional categories showed statistically significant differences with MG-RAST at KEGG level 2: in exacerbation, Cell growth and Death and Transport and Catabolism decreased in abundance [1. 6 (0. 2–2. 3) vs 3. 6 (3. 3–6. 9), p = 0. 012; and 1. 8 (0–3. 3) vs 3. 6 (1. 8–5. 1), p = 0. 025 respectively], while Cancer and Carbohydrate Metabolism increased [0. 8 (0–1. 5) vs 0 (0–0. 5), p = 0. 043; and 7 (6. 4–9) vs 5. 9 (6. 3–6. 1), p = 0. 012 respectively]. In conclusion, the bronchial microbiome as a whole is not significantly modified when exacerbation symptoms appear in severe COPD patients, but its functional metabolic capabilities show significant changes in several pathways.
Note: Altres ajuts: Sociedad Catalana de Neumología; Fundació Catalana de Neumología; Fundació Parc Tauli; Marató de TV3; Sociedad Española de Neumología y Cirugía Torácica; Fundación Menarini; Generalitat Valenciana (Spain) [Prometeo/2009/092] i Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES)
Note: Número d'acord de subvenció MINECO/SAF2012-31187
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Chronic obstructive pulmonary disease ; Microbiome ; Ribosomal RNA ; Sequence databases ; Carbohydrate metabolism ; Bacteria ; Metagenomics ; Sputum
Published in: PLoS One, Vol. 10, Issue 12 (December 2015) , p. e0144448, ISSN 1932-6203

DOI: 10.1371/journal.pone.0144448
PMID: 26632844

17 p, 2.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles

 Record created 2016-01-14, last modified 2018-10-28

   Favorit i Compartir