In Vivo and In Vitro characterization of a plasmodium liver stage-specific promoter
De Niz, Mariana (University of Bern (Suïssa))
Helm, Susanne (Bernhard-Nocht-Institut für Tropenmedizin)
Horstmann, Sebastian (Bernhard-Nocht-Institut für Tropenmedizin)
Annoura, Takeshi (Universiteit Leiden)
Portillo Obando, Hernando A. del (Centre de Recerca en Salut Internacional de Barcelona)
Khan, Shahid M. (Universiteit Leiden)
Heussler, Volker T. (University of Bern (Suïssa))
Institut Germans Trias i Pujol

Date:	2015
Abstract:	Little is known about stage-specific gene regulation in Plasmodium parasites, in particular the liver stage of development. We have previously described in the Plasmodium berghei rodent model, a liver stage-specific (lisp2) gene promoter region, in vitro. Using a dual luminescence system, we now confirm the stage specificity of this promoter region also in vivo. Furthermore, by substitution and deletion analyses we have extended our in vitro characterization of important elements within the promoter region. Importantly, the dual luminescence system allows analyzing promoter constructs avoiding mouse-consuming cloning procedures of transgenic parasites. This makes extensive mutation and deletion studies a reasonable approach also in the malaria mouse model. Stage-specific expression constructs and parasite lines are extremely valuable tools for research on Plasmodium liver stage biology. Such reporter lines offer a promising opportunity for assessment of liver stage drugs, characterization of genetically attenuated parasites and liver stage-specific vaccines both in vivo and in vitro, and may be key for the generation of inducible systems.
Grants:	European Commission 242095
Note:	Altres ajuts: SNF/310030_140691/1
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Luciferase ; Luminescence ; Plasmodium ; Parasitic diseases ; Promoter regions ; Sporozoites ; Blood ; Parasitic life cycles
Published in:	PloS one, Vol. 10 Núm. 4 (2015) , p. e0123473, ISSN 1932-6203

DOI: 10.1371/journal.pone.0123473
PMID: 25874388

17 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles