Production of lipases by solid state fermentation using vegetable oil-refining wastes
Santis Navarro, Angélica María (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Barrena Gómez, Raquel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Date: |
2011 |
Abstract: |
Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45 °C for 20 days) in 4. 5-L reactors. The lipases were extracted from the solid medium using 100 mM Tris-HCl, pH 8. 0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61-65 °C and at pH 7. 7-9. For the solid samples, the lipolytic activity reached up to 120,000 UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts. |
Grants: |
Ministerio de Ciencia e Innovación CTM2009-14073-C02-01
|
Rights: |
Tots els drets reservats. |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió sotmesa a revisió |
Subject: |
Solid-state fermentation ;
Lipase ;
Thermostability ;
Organic wastes ;
Sewage sludge |
Published in: |
Bioresource technology, Vol. 102, Issue 21 (Nov. 2011) , p. 10080-10084, ISSN 0960-8524 |
DOI: 10.1016/j.biortech.2011.08.062
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Record created 2016-09-12, last modified 2023-03-09