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Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential : a molecular and clinicopathological study
Gonzalo Calvo, David de (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
López Vilaró, Laura (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Nasarre, Laura (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Perez Olabarria, Maitane (Institut d'Investigacions Biomèdiques Sant Pau)
Vázquez, Tania (Institut d'Investigacions Biomèdiques Sant Pau)
Escuin i Borràs, Daniel (Institut d'Investigacions Biomèdiques Sant Pau)
Badimón Maestro, Lina (Institut de Ciències Cardiovasculars de Catalunya)
Barnadas Molins, Agustí (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya). Servei d'Oncologia Mèdica)
Lerma Puertas, Enrique (Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques)
Llorente Cortés, Vicenta (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))

Date: 2015
Abstract: Background: The metabolic effect of intratumor cholesteryl ester (CE) in breast cancer remains poorly understood. The objective was to analyze the relationship between intratumor CE content and clinicopathological variables in human breast carcinomas. Methods: We classified 30 breast carcinoma samples into three subgroups: 10 luminal-A tumors (ER+/PR+/Her2-), 10 Her-2 tumors (ER-/PR-/Her2+), and 10 triple negative (TN) tumors (ER-/PR-/Her2-). We analyzed intratumor neutral CE, free cholesterol (FC) and triglyceride (TG) content by thin layer chromatography after lipid extraction. RNA and protein levels of lipid metabolism and invasion mediators were analyzed by real time PCR and Western blot analysis. Results: Group-wise comparisons, linear regression and logistic regression models showed a close association between CE-rich tumors and higher histologic grade, Ki-67 and tumor necrosis. CE-rich tumors displayed higher mRNA and protein levels of low-density lipoprotein receptor (LDLR) and scavenger receptor class B member 1 (SCARB1). An increased expression of acetyl-Coenzyme A acetyltransferase 1 (ACAT1) in CE-rich tumors was also reported. Conclusions: Intratumor CE accumulation is intimately linked to proliferation and aggressive potential of breast cancer tumors. Our data support the link between intratumor CE content and poor clinical outcome and open the door to new antitumor interventions.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: ACAT1 ; Breast cancer ; CD36 ; Cholesteryl ester ; LDL receptor ; SCARB1
Published in: BMC Cancer, Vol. 15 N. 460 (June 2015) , p. 1-13, ISSN 1471-2407

DOI: 10.1186/s12885-015-1469-5
PMID: 26055977

14 p, 2.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació Biomèdica Sant Pau
Articles > Research articles
Articles > Published articles

 Record created 2017-03-08, last modified 2019-07-21

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