Web of Science: 2 citations, Scopus: 2 citations, Google Scholar: citations
Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival.
Blancas, Sugela (Universidad Nacional Autónoma de México. Instituto de Fisiología Celular)
Fadó Andrés, Rut (Universitat Autònoma de Barcelona. Institut de Neurociències)
Rodríguez Álvarez, José (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Morán, Julio (Universidad Nacional Autónoma de México. Instituto de Fisiología Celular)

Date: 2014
Abstract: Programmed cell death plays a critical role during cerebellar development. In particular, it has been shown in vivo and in vitro that developing cerebellar granule neurons (CGN) die apoptotically. Apoptosis involves a series of morphological changes and the activation of caspases. Inhibitor of apoptosis proteins (IAPs) is implicated in negative regulation of caspase activation and apoptotic cell death. Although apoptotic death of CGN has been extensively studied, there is no information about the role of IAPs in the developing cerebellum. Here, we studied the participation of some members of IAPs in the survival of the developing rat CGN in culture and under physiological conditions. Under these conditions, we found a differential expression pattern of cIAP-1, cIAP-2, XIAP and survivin during cerebellar development in an age-dependent manner, highlighting the significant increase of XIAP levels. We also detected an interaction between XIAP and caspase 3 at postnatal day (P) 12 and 16. On the other hand, we found a significant decrease of XIAP levels in cultured CGN maintained in chronic potassium deprivation, an apoptotic condition, suggesting a possible relationship between XIAP levels and neuronal viability. Under these conditions, we also detected the interaction of XIAP with active caspase-3. The down-regulation of XIAP in CGN cultured under survival conditions (chronic potassium depolarization) induced a reduction of cell viability and an increment of apoptotic cells. These findings support the idea that IAPs could be involved in the survival of CGN and that XIAP might be critical for neuronal survival in cerebellar development and during chronic depolarization in cultured CGN through a mechanism involving caspase inhibition.
Note: Altres ajuts: DGAPA-PAPIIT-UNAM (IN206213) i CONACYT (179234)
Note: Número d'acord de subvenció MICINN/SAF2008-01904
Note: Número d'acord de subvenció CIBERNED/CB06/05/0042
Note: Número d'acord de subvenció ISCIII/RD06/0026/1009
Note: Número d'acord de subvenció AGAUR/SGR2009-1231
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Apoptotic-like death ; IAPs ; Caspases ; Cerebellum development ; Cerebellar granule neurons ; Potassium deprivation
Published in: International Journal of Developmental Neuroscience, Vol. 37 (October 2014) , p. 26-35, ISSN 1873-474X

DOI: 10.1016/j.ijdevneu.2014.06.006


Post-print
34 p, 2.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles

 Record created 2017-03-24, last modified 2018-07-24



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