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Pàgina inicial > Articles > Articles publicats > Production of cecropin A antimicrobial peptide in rice seed endosperm |
Data: | 2014 |
Resum: | Background: Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. - Results: transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. - Conclusions: rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation. |
Ajuts: | Ministerio de Ciencia e Innovación EUI2008-03769 Ministerio de Ciencia e Innovación EUI2008-03572 Ministerio de Ciencia e Innovación CSD2007-00036 Agència de Gestió d'Ajuts Universitaris i de Recerca 2008-SGR-812 Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-09626 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Rice ; Antimicrobial peptides ; Cecropin A ; Endosperm ; Protein bodies ; Pathogen resistance ; Fusarium verticillioides ; Dickeya dadantii ; Oryza sativa |
Publicat a: | BMC plant biology, Vol. 14 (April 2014) , art 102, ISSN 1471-2229 |
13 p, 4.1 MB |