The Arabidopsis miR396 mediates pathogen-associated molecular pattern-triggered immune responses against fungal pathogens
Soto-Suárez, Mauricio (Centre de Recerca en Agrigenòmica)
Baldrich, Patricia (Centre de Recerca en Agrigenòmica)
Weigel, Detlef (Max Planck Institute for Developmental Biology)
Rubio-Somoza, Ignacio (Centre de Recerca en Agrigenòmica)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Data:	2017
Resum:	MicroRNAs (miRNAs) play a pivotal role in regulating gene expression during plant development. Although a substantial fraction of plant miRNAs has proven responsive to pathogen infection, their role in disease resistance remains largely unknown, especially during fungal infections. In this study, we screened Arabidopsis thaliana lines in which miRNA activity has been reduced using artificial miRNA target mimics (MIM lines) for their response to fungal pathogens. Reduced activity of miR396 (MIM396 plants) was found to confer broad resistance to necrotrophic and hemibiotrophic fungal pathogens. MiR396 levels gradually decreased during fungal infection, thus, enabling its GRF (GROWTH-REGULATING FACTOR) transcription factor target genes to trigger host reprogramming. Pathogen resistance in MIM396 plants is based on a superactivation of defense responses consistent with a priming event during pathogen infection. Notably, low levels of miR396 are not translated in developmental defects in absence of pathogen challenge. Our findings support a role of miR396 in regulating plant immunity, and broaden our knowledge about the molecular players and processes that sustain defense priming. That miR396 modulates innate immunity without growth costs also suggests fine-tuning of miR396 levels as an effective biotechnological means for protection against pathogen infection.
Ajuts:	European Commission 340602 Ministerio de Economía y Competitividad BIO2015-67212-R Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BFU2014-58361-JIN Ministerio de Economía y Competitividad BIO2012-32838 Ministerio de Ciencia e Innovación BES-2010-032879
Nota:	Altres ajuts: Max Planck Society, DFG (grant SFB1101)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biotic ; Molecular engineering in plants
Publicat a:	Scientific reports, Vol. 7 (March 2017) , art. 44898, ISSN 2045-2322

DOI: 10.1038/srep44898
PMID: 28332603

14 p, 1.9 MB

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