TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana
Espinosa-Ruiz, Ana (Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas)
Martínez, Cristina (Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas)
Lucas, Miguel de (Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas)
Fábregas, Norma (Centre de Recerca en Agrigenòmica)
Bosch, Nadja (Centre de Recerca en Agrigenòmica)
Caño Delgado, Ana I. (Centre de Recerca en Agrigenòmica)
Prat, Salomé (Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas)

Date:	2017
Abstract:	The transcription factor BRI1-EMS-SUPRESSOR 1 (BES1) is a master regulator of brassinosteroid (BR)-regulated gene expression. BES1 together with BRASSINAZOLE-RESISTANT 1 (BZR1) drive activated or repressed expression of several genes, and have a prominent role in negative regulation of BR synthesis. Here, we report that BES1 interaction with TOPLESS (TPL), via its ERF-associated amphiphilic repression (EAR) motif, is essential for BES1-mediated control of organ boundary formation in the shoot apical meristem and the regulation of quiescent center (QC) cell division in roots. We show that TPL binds via BES1 to the promoters of the CUC3 and BRAVO targets and suppresses their expression. Ectopic expression of TPL leads to similar organ boundary defects and alterations in QC cell division rate to the bes1-d mutation, while bes1-d defects are suppressed by the dominant interfering protein encoded by tpl-1, with these effects respectively correlating with changes in CUC3 and BRAVO expression. Together, our data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems, which relies on its interaction with BES1 and regulation of BES1 target gene expression.
Grants:	European Commission 683163 Ministerio de Economía y Competitividad BIO2014-60064 Ministerio de Economía y Competitividad BIO2013-43873 Ministerio de Economía y Competitividad BIO2011-30546
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	BES1 ; BR signaling ; EAR domain ; Organ boundary ; Quiescence ; Quiescent center ; TOPLESS
Published in:	Development (Cambridge), Vol. 144 Issue 9 (May 2017) , p. 1619-1628, ISSN 1477-9129

DOI: 10.1242/dev.143214

10 p, 6.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles