DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis
Gallemí, Marçal (Centre de Recerca en Agrigenòmica)
Galstyan, Anahit (Centre de Recerca en Agrigenòmica)
Paulišić, Sandi (Centre de Recerca en Agrigenòmica)
Then, Christiane (Centre de Recerca en Agrigenòmica)
Ferrández-Ayela, Almudena (Universidad Miguel Hernández de Elche. Instituto de Bioingeniería)
Lorenzo-Orts, Laura (Centre de Recerca en Agrigenòmica)
Roig Villanova, Irma (Centre de Recerca en Agrigenòmica)
Wang, Xuewen (University of London. Royal Holloway. School of Biological Sciences)
Micol, Jose Luis (Universidad Miguel Hernández de Elche. Instituto de Bioingeniería)
Ponce, Maria Rosa (Universidad Miguel Hernández de Elche. Instituto de Bioingeniería)
Devlin, Paul F. (University of London. Royal Holloway. School of Biological Sciences)
Martínez García, Jaime F. (Centre de Recerca en Agrigenòmica)

Data:	2016
Resum:	When plants grow in close proximity basic resources such as light can become limiting. Under such conditions plants respond to anticipate and/or adapt to the light shortage, a process known as the shade avoidance syndrome (SAS). Following genetic screening using a shade-responsive luciferase reporter line (PHYB:LUC), we identified DRACULA2 (DRA2), which encodes an Arabidopsis homolog of mammalian nucleoporin 98, a component of the nuclear pore complex (NPC). DRA2, together with other nucleoporins, participates positively in the control of the hypocotyl elongation response to plant proximity, a role that can be considered dependent on the nucleocytoplasmic transport of macromolecules (i. e. is transport dependent). In addition, our results reveal a specific role for DRA2 in controlling shade-induced gene expression. We suggest that this novel regulatory role of DRA2 is transport independent and that it might rely on its dynamic localization within and outside of the NPC. These results provide mechanistic insights in to how SAS responses are rapidly established by light conditions. They also indicate that nucleoporins have an active role in plant signaling.
Ajuts:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BIO2014-59895-P Ministerio de Economía y Competitividad BIO2011-23489 Agència de Gestió d'Ajuts Universitaris i de Recerca 2011/SGR-44 Ministerio de Economía y Competitividad BIO2008-00169
Nota:	Altres ajuts: C.T. received a Marie Curie IEF postdoctoral contract funded by the European Commission.I.R.-V. received initiallyan FPIfellowship from the SpanishMINECO and later a Beatriu de Pinós contract from AGAUR. - Our research is supported by grants from Generalitat Valenciana [PROMETEO/2009/112, PROMETEOII/2014/006] to M.R.P. and J.L.M.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Arabidopsis thaliana ; Hypocotyl elongation ; Nucleoporin ; Nup98 ; Shade avoidance syndrome ; Shade-induced gene expression
Publicat a:	Development (Cambridge), Vol. 143, issue 9 (May 2016) , p. 1623-1631, ISSN 1477-9129

DOI: 10.1242/dev.130211

9 p, 3.0 MB

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