Home > Articles > Published articles > A non-DNA-binding activity for the ATHB4 transcription factor in the control of vegetation proximity |
Date: | 2017 |
Abstract: | In plants, perception of vegetation proximity by phytochrome photoreceptors activates a transcriptional network that implements a set of responses to adapt to plant competition, including elongation of stems or hypocotyls. In Arabidopsis thaliana, the homeodomain-leucine zipper (HD-Zip) transcription factor ARABIDOPSIS THALIANA HOMEOBOX 4 (ATHB4) regulates this and other responses, such as leaf polarity. To better understand the shade regulatory transcriptional network, we have carried out structure-function analyses of ATHB4 by overexpressing a series of truncated and mutated forms and analyzing three different responses: hypocotyl response to shade, transcriptional activity and leaf polarity. Our results indicated that ATHB4 has two physically separated molecular activities: that performed by HD-Zip, which is involved in binding to DNA-regulatory elements, and that performed by the ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR-associated amphiphilic repression (EAR)-containing N-terminal region, which is involved in protein-protein interaction. Whereas both activities are required to regulate leaf polarity, DNA-binding activity is not required for the regulation of the seedling responses to plant proximity, which indicates that ATHB4 works as a transcriptional cofactor in the regulation of this response. These findings suggest that transcription factors might employ alternative mechanisms of action to regulate different developmental processes. |
Grants: | Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BIO2014-59895-P Ministerio de Economía y Competitividad BIO2011-23489 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-447 |
Note: | Altres ajuts: Generalitat de Catalunya through the CERCA Program |
Rights: | Tots els drets reservats. |
Language: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Subject: | Arabidopsis thaliana ; Arabidopsis thaliana homeobox 4 (ATHB4) ; DNA-binding activity ; Ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif ; Homeodomain-leucine zipper ; Shade avoidance syndrome |
Published in: | The new phytologist, Vol. 216, issue 3 (Nov. 2017) , p. 798-813, ISSN 1469-8137 |
Post-print. Article 41 p, 15.2 MB |
Post-print. Supplementary material 29 p, 2.2 MB |