A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
Diaz, Aurora (Universitat Politècnica de València. Instituto de Biología Molecular y Celular de Plantas)
Fergany, Mohamed (Centre de Recerca en Agrigenòmica)
Formisano, Gelsomina (Università di Napoli. Dipartimento del suolo, della pianta, dell'ambiente e dell'animale Scienze della produzione)
Ziarsolo, Peio (Universitat Politècnica de València. Istituto per la conservazione e l'allevamento della biodiversità agricola)
Blanca, José (Universitat Politècnica de València. Istituto per la conservazione e l'allevamento della biodiversità agricola)
Fei, Zhanjun (Boyce Thompson Institute for Plant Research)
Staub, Jack E. (United States. Agricultural Research Service. Vegetable Crops Research Unit)
Zalapa, Juan (United States. Agricultural Research Service. Vegetable Crops Research Unit)
Cuevas, Hugo E. (United States. Agricultural Research Service. Vegetable Crops Research Unit)
Dace, Gayle (Syngenta Biotechnology)
Oliver, Marc (Syngenta Seeds)
Boissot, Nathalie (Institut national de la recherche agronomique (France). Unité de Génétique et d'Amélioration des Fruits et Légume)
Dogimont, Catherine (Institut national de la recherche agronomique (France). Unité de Génétique et d'Amélioration des Fruits et Légume)
Pitrat, Michel (Institut national de la recherche agronomique (France). Unité de Génétique et d'Amélioration des Fruits et Légume)
Hofstede, René (Keygene N.V.)
Van Koert, Paul (Keygene N.V.)
Harel-Beja, Rotem (Agricultural Research Organization. Institute of Plant Science)
Tzuri, Galil (Agricultural Research Organization. Institute of Plant Science)
Portnoy, Vitaly (Agricultural Research Organization. Institute of Plant Science)
Cohen, Shahar (Institute of Plant Science. Agricultural Research Organization)
Schaffer, Arthur (Institute of Plant Science. Agricultural Research Organization)
Katzir, Nurit (Agricultural Research Organization. Institute of Plant Science)
Xu, Yong (National Engineering Research Center for Vegetables)
Zhang, Haiying (National Engineering Research Center for Vegetables)
Fukino, Nobuko (Yasai Chagyō Kenkyūjo (Japan))
Matsumoto, Satoru (Yasai Chagyō Kenkyūjo (Japan))
Garcia Mas, Jordi (Centre de Recerca en Agrigenòmica)
Monforte, Antonio J. (Centre de Recerca en Agrigenòmica)

Date: 2011
Abstract: A number of molecular marker linkage maps have been developed for melon (Cucumis melo L. ) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via markerassisted selection (MAS). - Results: Under the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www. icugi. org), an integrated genetic map has been constructed by merging data from eight independent mapping experiments using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels and 11 morphological traits) with a mean marker density of 0. 72 cM/marker. One hundred and ninety-six of these markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map. Some QTL associated with economically important traits detected in separate studies mapped to similar genòmic positions. For example, independently identified QTL controlling fruit shape were mapped on similar genòmic positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in a broad array of melon germplasm. - Conclusions: Even though relatively unsaturated genetic maps in a diverse set of melon market types have been published, the integrated saturated map presented herein should be considered the initial reference map for melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e. g. , integration of physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted selection).
Note: Número d'acord de subvenció MICINN/AGL2009/12698-C02-02
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: BMC plant biology, Vol. 11 (Jul. 2011) , art. 111, ISSN 1471-2229

PMID: 21797998


14 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-08-30, last modified 2019-02-15



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