Web of Science: 17 citations, Scopus: 18 citations, Google Scholar: citations,
The antiphasic regulatory module comprising CDF5 and its antisense RNA FLORE links the circadian clock to photoperiodic flowering
Henriques, Rossana (Rockefeller University. Laboratory of Plant Molecular Biology)
Wang, Huan (The Rockefeller University. Laboratory of Plant Molecular Biology)
Liu, Jun (The Rockefeller University. Laboratory of Plant Molecular Biology)
Boix, Marc (Centre de Recerca en Agrigenòmica)
Huang, Li-Fang (The Rockefeller University. Laboratory of Plant Molecular Biology)
Chua, Nam-Hai (The Rockefeller University. Laboratory of Plant Molecular Biology)

Date: 2017
Abstract: Circadian rhythms of gene expression are generated by the combinatorial action of transcriptional and translational feedback loops as well as chromatin remodelling events. Recently, long noncoding RNAs (lncRNAs) that are natural antisense transcripts (NATs) to transcripts encoding central oscillator components were proposed as modulators of core clock function in mammals (Per) and fungi (frq/qrf). Although oscillating lncRNAs exist in plants, their functional characterization is at an initial stage. By screening an Arabidopsis thaliana lncRNA custom-made array we identified CDF5 LONG NONCODING RNA (FLORE), a circadian-regulated lncRNA that is a NAT of CDF5. Quantitative real-time RT-PCR confirmed the circadian regulation of FLORE, whereas GUS-staining and flowering time evaluation were used to determine its biological function. FLORE and CDF5 antiphasic expression reflects mutual inhibition in a similar way to frq/qrf. Moreover, whereas the CDF5 protein delays flowering by directly repressing FT transcription, FLORE promotes it by repressing several CDFs (CDF1, CDF3, CDF5) and increasing FT transcript levels, indicating both cis and trans function. We propose that the CDF5/FLORE NAT pair constitutes an additional circadian regulatory module with conserved (mutual inhibition) and unique (function in trans) features, able to fine-tune its own circadian oscillation, and consequently, adjust the onset of flowering to favourable environmental conditions.
Note: Número d'acord de subvenció EC/FP7/334052
Note: Número d'acord de subvenció MINECO/BIO2015-70812-ERC
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Rights: Tots els drets reservats
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Circadian clock ; CYCLING DOF FACTORs (CDFs) ; Flowering time ; Long noncoding RNA ; Natural antisense transcripts
Published in: New phytologist, Vol. 216, issue 3 (Nov. 2017) , p. 854–867, ISSN 0028-646X

DOI: 10.1111/nph.14703


Post-print. Article
38 p, 2.0 MB

Post-print. Material addicional
17 p, 1.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-09-07, last modified 2019-02-02



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