The antiphasic regulatory module comprising CDF5 and its antisense RNA FLORE links the circadian clock to photoperiodic flowering
Henriques, Rossana (Rockefeller University. Laboratory of Plant Molecular Biology)
Wang, Huan (The Rockefeller University. Laboratory of Plant Molecular Biology)
Liu, Jun (The Rockefeller University. Laboratory of Plant Molecular Biology)
Boix, Marc (Centre de Recerca en Agrigenòmica)
Huang, Li-Fang (The Rockefeller University. Laboratory of Plant Molecular Biology)
Chua, Nam-Hai (The Rockefeller University. Laboratory of Plant Molecular Biology)

Data:	2017
Resum:	Circadian rhythms of gene expression are generated by the combinatorial action of transcriptional and translational feedback loops as well as chromatin remodelling events. Recently, long noncoding RNAs (lncRNAs) that are natural antisense transcripts (NATs) to transcripts encoding central oscillator components were proposed as modulators of core clock function in mammals (Per) and fungi (frq/qrf). Although oscillating lncRNAs exist in plants, their functional characterization is at an initial stage. By screening an Arabidopsis thaliana lncRNA custom-made array we identified CDF5 LONG NONCODING RNA (FLORE), a circadian-regulated lncRNA that is a NAT of CDF5. Quantitative real-time RT-PCR confirmed the circadian regulation of FLORE, whereas GUS-staining and flowering time evaluation were used to determine its biological function. FLORE and CDF5 antiphasic expression reflects mutual inhibition in a similar way to frq/qrf. Moreover, whereas the CDF5 protein delays flowering by directly repressing FT transcription, FLORE promotes it by repressing several CDFs (CDF1, CDF3, CDF5) and increasing FT transcript levels, indicating both cis and trans function. We propose that the CDF5/FLORE NAT pair constitutes an additional circadian regulatory module with conserved (mutual inhibition) and unique (function in trans) features, able to fine-tune its own circadian oscillation, and consequently, adjust the onset of flowering to favourable environmental conditions.
Ajuts:	European Commission 334052 Ministerio de Economía y Competitividad BIO2015-70812-ERC Ministerio de Economía y Competitividad SEV-2015-0533
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Circadian clock ; CYCLING DOF FACTORs (CDFs) ; Flowering time ; Long noncoding RNA ; Natural antisense transcripts
Publicat a:	The new phytologist, Vol. 216, issue 3 (Nov. 2017) , p. 854-867, ISSN 1469-8137

DOI: 10.1111/nph.14703

Post-print. Article 38 p, 2.0 MB

Post-print. Material addicional 17 p, 1.5 MB

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