Web of Science: 14 citations, Scopus: 15 citations, Google Scholar: citations,
A mutation in the melon vacuolar protein sorting 41prevents systemic infection of cucumber mosaic virus
Giner, Ana (Centre de Recerca en Agrigenòmica)
Pascual, Laura (Centre de Recerca en Agrigenòmica)
Bourgeois, Michael (Centre de Recerca en Agrigenòmica)
Gyetvai, Gabor (Centre de Recerca en Agrigenòmica)
Ríos Rodríguez, Pablo (Centre de Recerca en Agrigenòmica)
Picó, Belén (Centro de Conservación y Mejora de la Agrodiversidad Valenciana)
Troadec,Christelle (Institute of Plant Sciences Paris-Saclay)
Bendahmane, Abdel (Institute of Plant Sciences Paris-Saclay)
Garcia Mas, Jordi (Centre de Recerca en Agrigenòmica)
Martín-Hernández, Ana Montserrat (Centre de Recerca en Agrigenòmica)

Date: 2017
Abstract: In the melon exotic accession PI 161375, the gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents the systemic infection by restricting the virus to the bundle sheath cells and impeding viral loading to the phloem. Here we report the fine mapping and cloning of cmv1. Screening of an F2 population reduced the cmv1 region to a 132 Kb interval that includes a Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals and yeast and is required for post-Golgi vesicle trafficking towards the vacuole. We have validated CmVPS41 as the gene responsible for the resistance, both by generating CMV susceptible transgenic melon plants, expressing the susceptible allele in the resistant cultivar and by characterizing CmVPS41 TILLING mutants with reduced susceptibility to CMV. Finally, a core collection of 52 melon accessions allowed us to identify a single amino acid substitution (L348R) as the only polymorphism associated with the resistant phenotype. CmVPS41 is the first natural recessive resistance gene found to be involved in viral transport and its cellular function suggests that CMV might use CmVPS41 for its own transport towards the phloem.
Note: Número d'acord de subvenció EC/FP7/341076
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Note: Número d'acord de subvenció MINCINN/AGL2012-40130-C02-01
Note: Número d'acord de subvenció MINCINN/AGL2009-12698-C02-01
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Biotic ; Protein trafficking
Published in: Scientific reports, Vol. 7 (Sep. 2017) , art. 10471, ISSN 2045-2322

DOI: 10.1038/s41598-017-10783-3
PMID: 28874719


12 p, 1.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-09-22, last modified 2020-02-29



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