The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca
Bonet Gigante, Julio (Centre de Recerca en Agrigenòmica)
López Girona, Elena (East Malling Research)
Sargent, Daniel J. (East Malling Research)
Muñoz Torres, Monica C. (Clemson University. Department of Genetics and Biochemistry)
Monfort, Amparo (Centre de Recerca en Agrigenòmica)
Abbott, Albert G. (Clemson University. Department of Genetics and Biochemistry)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)
Simpson, David W. (East Malling Research)
Davik, Jahn (Bioforsk Midt-Norge)

Date:	2009
Abstract:	BACKGROUND: the cultivated strawberry Fragaria xananassa is one of the most economically-important soft-fruit species. Few structural genomic resources have been reported for Fragaria and there exists an urgent need for the development of physical mapping resources for the genus. The first stage in the development of a physical map for Fragaria is the construction and characterisation of a high molecular weight bacterial artificial chromosome (BAC) library. - METHODS: a BAC library, consisting of 18,432 clones was constructed from Fragaria vesca f. semperflorens accession 'Ali Baba'. BAC DNA from individual library clones was pooled to create a PCR-based screening assay for the library, whereby individual clones could be identified with just 34 PCR reactions. These pools were used to screen the BAC library and anchor individual clones to the diploid Fragaria reference map (FVxFN). - FINDINGS: clones from the BAC library developed contained an average insert size of 85 kb, representing over seven genome equivalents. The pools and superpools developed were used to identify a set of BAC clones containing 70 molecular markers previously mapped to the diploid Fragaria FVxFN reference map. The number of positive colonies identified for each marker suggests the library represents between 4x and 10x coverage of the diploid Fragaria genome, which is in accordance with the estimate of library coverage based on average insert size. - CONCLUSION: this BAC library will be used for the construction of a physical map for F. vesca and the superpools will permit physical anchoring of molecular markers using PCR.
Grants:	European Commission COST action 863 Ministerio de Ciencia e Innovación INIA/RTA2007-00063-00-00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2006/FI00408
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	BMC research notes, Vol. 2 (Set. 2009) , art. 188, ISSN 1756-0500

DOI: 10.1186/1756-0500-2-188
PMID: 19772672

5 p, 238.6 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles