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QTL analyses in multiple populations employed for the fine mapping and identification of candidate genes at a locus affecting sugar accumulation in melon (Cucumis melo L.)
Argyris, Jason (Centre de Recerca en Agrigenòmica)
Díaz, Aurora (Universitat Politècnica de València. Instituto de Biología Molecular y Celular de Plantas)
Ruggieri, Valentino (Centre de Recerca en Agrigenòmica)
Fernández, Marta (Semillas Fitó, S.L.)
Jahrmann, Torben (Semillas Fitó, S.L.)
Gibon, Yves (Institut national de la recherche agronomique (França))
Picó, Belén (Universitat Politècnica de València. Instituto de Conservación y Mejora de la Agrodiversidad Valenciana)
Martín-Hernández, Ana Montserrat (Centre de Recerca en Agrigenòmica)
Monforte, Antonio J. (Universitat Politècnica de València. Instituto de Biología Molecular y Celular de Plantas)
Garcia Mas, Jordi (Centre de Recerca en Agrigenòmica)

Date: 2017
Abstract: Sugar content is the major determinant of both fruit quality and consumer acceptance in melon (Cucumis melo L), and is a primary target for crop improvement. Near-isogenic lines (NILs) derived from the intraspecific cross between a "Piel de Sapo" (PS) type and the exotic cultivar "Songwhan Charmi" (SC), and several populations generated from the cross of PS × Ames 24294 ("Trigonus"), a wild melon, were used to identify QTL related to sugar and organic acid composition. Seventy-eight QTL were detected across several locations and different years, with three important clusters related to sugar content located on chromosomes 4, 5, and 7. Two PS × SC NILs (SC5-1 and SC5-2) sharing a common genomic interval of 1. 7 Mb at the top of chromosome 5 contained QTL reducing soluble solids content (SSC) and sucrose content by an average of 29 and 68%, respectively. This cluster collocated with QTL affecting sugar content identified in other studies in lines developed from the PS × SC cross and supported the presence of a stable consensus locus involved in sugar accumulation that we named SUCQSC5. 1. QTL reducing soluble solids and sucrose content identified in the "Trigonus" mapping populations, as well as QTL identified in previous studies from other ssp. agrestis sources, collocated with SUCQSC5. 1, suggesting that they may be allelic and implying a role in domestication. In subNILs derived from the PS × SC5-1 cross, SUCQSC5. 1 reduced SSC and sucrose content by an average of 18 and 34%, respectively, and was fine-mapped to a 56. 1 kb interval containing four genes. Expression analysis of the candidate genes in mature fruit showed differences between the subNILs with PS alleles that were "high" sugar and SC alleles of "low" sugar phenotypes for MELO3C014519, encoding a putative BEL1-like homeodomain protein. Sequence differences in the gene predicted to affect protein function were restricted to SC and other ssp. agrestis cultivar groups. These results provide the basis for further investigation of genes affecting sugar accumulation in melon.
Note: Número d'acord de subvenció MINECO/AGL2015-64625-C2-1-R
Note: Número d'acord de subvenció MINECO/AGL2015-64625-C2-R
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Note: Número d'acord de subvenció MINECO/PIM2010PKB-00691
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: QTL ; Melon ; Sugar ; Sucrose ; NILs ; Fine-mapping ; Candidate genes ; BEL1-like
Published in: Frontiers in plant science, Vol. 8 (Sep. 2017) art. 1679, ISSN 1664-462X

DOI: 10.3389/fpls.2017.01679
PMID: 29018473

20 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-11-28, last modified 2019-11-27

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