Web of Science: 5 citas, Scopus: 5 citas, Google Scholar: citas,
Type III secretion-dependent and -independent phenotypes caused by Ralstonia solanacearum in Arabidopsis Roots
Lu, Haibin (Centre de Recerca en Agrigenòmica)
Lema, Saul (Centre de Recerca en Agrigenòmica)
Planas Marquès, Marc (Centre de Recerca en Agrigenòmica)
Alonso-Díaz, Alejandro (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Coll, Núria (Centre de Recerca en Agrigenòmica)

Fecha: 2018
Resumen: The causal agent of bacterial wilt, Ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. The effects of R. solanacearum infection are devastating, and no effective approach to fight the disease is so far available. The early steps of infection, essential for colonization, as well as the early plant defense responses remain mostly unknown. Here, we have set up a simple, in vitro Arabidopsis thaliana-R. solanacearum pathosystem that has allowed us to identify three clear root phenotypes specifically associated to the early stages of infection: root-growth inhibition, root-hair formation, and root-tip cell death. Using this method, we have been able to differentiate, on Arabidopsis plants, the phenotypes caused by mutants in the key bacterial virulence regulators hrpB and hrpG, which remained indistinguishable using the classical soil-drench inoculation pathogenicity assays. In addition, we have revealed the previously unknown involvement of auxins in the root rearrangements caused by R. solanacearum infection. Our system provides an easy-to-use, high-throughput tool to study R. solanacearum aggressiveness. Furthermore, the observed phenotypes may allow the identification of bacterial virulence determinants and could even be used to screen for novel forms of early plant resistance to bacterial wilt.
Nota: Altres ajuts: BP_B 00030 (Generalitat de Catalunya); COST Action SUSTAIN (FA1208); Programa CERCA (Generalitat de Catalunya)
Nota: Número d'acord de subvenció EC/FP7/331392
Nota: Número d'acord de subvenció EC/FP7/321738
Nota: Número d'acord de subvenció MINECO/AGL2016-78002-R
Nota: Número d'acord de subvenció MINECO/RyC 2014-1658
Nota: Número d'acord de subvenció MINECO/SEV-2015-0533
Nota: Número d'acord de subvenció MINECO/AGL2013-46898-R
Derechos: Tots els drets reservats
Lengua: Anglès
Documento: article ; recerca ; acceptedVersion
Materia: Arabidopsis thaliana ; Bacterial wilt ; Cell death ; In vitro 49 pathosystem ; Ralstonia solanacearum ; Root hair ; Root growth
Publicado en: Molecular plant-microbe interactions, Vol. 31, no. 1 (Jan. 2018) , p. 175-184, ISSN 0894-0282

DOI: 10.1094/MPMI-05-17-0109-FI

34 p, 5.5 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2017-11-30, última modificación el 2020-08-09

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