Type III secretion-dependent and -independent phenotypes caused by Ralstonia solanacearum in Arabidopsis Roots
Lu, Haibin (Centre de Recerca en Agrigenòmica)
Lema A., Saul (Centre de Recerca en Agrigenòmica)
Planas-Marquès, Marc (Centre de Recerca en Agrigenòmica)
Alonso-Díaz, Alejandro (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Data:	2018
Resum:	The causal agent of bacterial wilt, Ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. The effects of R. solanacearum infection are devastating, and no effective approach to fight the disease is so far available. The early steps of infection, essential for colonization, as well as the early plant defense responses remain mostly unknown. Here, we have set up a simple, in vitro Arabidopsis thaliana-R. solanacearum pathosystem that has allowed us to identify three clear root phenotypes specifically associated to the early stages of infection: root-growth inhibition, root-hair formation, and root-tip cell death. Using this method, we have been able to differentiate, on Arabidopsis plants, the phenotypes caused by mutants in the key bacterial virulence regulators hrpB and hrpG, which remained indistinguishable using the classical soil-drench inoculation pathogenicity assays. In addition, we have revealed the previously unknown involvement of auxins in the root rearrangements caused by R. solanacearum infection. Our system provides an easy-to-use, high-throughput tool to study R. solanacearum aggressiveness. Furthermore, the observed phenotypes may allow the identification of bacterial virulence determinants and could even be used to screen for novel forms of early plant resistance to bacterial wilt.
Ajuts:	European Commission 331392 European Commission 321738 Ministerio de Economía y Competitividad AGL2016-78002-R Ministerio de Economía y Competitividad RyC 2014-1658 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2013-46898-R
Nota:	Altres ajuts: BP_B 00030 (Generalitat de Catalunya); COST Action SUSTAIN (FA1208); Programa CERCA (Generalitat de Catalunya)
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Arabidopsis thaliana ; Bacterial wilt ; Cell death ; In vitro 49 pathosystem ; Ralstonia solanacearum ; Root hair ; Root growth
Publicat a:	Molecular plant-microbe interactions, Vol. 31, no. 1 (Jan. 2018) , p. 175-184, ISSN 0894-0282

DOI: 10.1094/MPMI-05-17-0109-FI
PMID: 28840786

Post-print 34 p, 5.5 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats