QDs versus Alexa : reality of promising tools for immunocytochemistry
Montón i Domingo, Helena (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Nogués, Carme (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Rossinyol Casals, Emma (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Castell, Onofre (Universitat Autònoma de Barcelona. Servei de Microscòpia)
Roldán, Mónica (Universitat Autònoma de Barcelona. Servei de Microscòpia)

Data:	2009
Resum:	The unique photonic properties of the recently developed fluorescent semiconductor nanocrystals (QDs) have made them a potential tool in biological research. However, QDs are not yet a part of routine laboratory techniques. Double and triple immunocytochemistries were performed in HeLa cell cultures with commercial CdSe QDs conjugated to antibodies. The optical characteristics, due to which QDs can be used as immunolabels, were evaluated in terms of emission spectra, photostability and specificity. QDs were used as secondary and tertiary antibodies to detect β-tubulin (microtubule network), GM130 (Golgi complex) and EEA1 (endosomal system). The data obtained were compared to homologous Alexa Fluor 594 organic dyes. It was found that QDs are excellent fluorochromes with higher intensity, narrower bandwidth values and higher photostability than Alexa dyes in an immunocytochemical process. In terms of specificity, QDs showed high specificity against GM130 and EEA1 primary antibodies, but poor specificity against β-tubulin. Alexa dyes showed good specificity for all the targets tested. This study demonstrates the great potential of QDs, as they are shown to have superior properties to Alexa dyes. Although their specificity still needs to be improved in some cases, QDs conjugated to antibodies can be used instead of organic molecules in routine immunocytochemistry.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Journal of nanobiotechnology, Vol. 7 (May 2009) , art. 4, ISSN 1477-3155

DOI: 10.1186/1477-3155-7-4
PMID: 19473541

10 p, 4.0 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats