The addition of reduced glutathione to cryopreservation media induces changes in the structure of motile subpopulations of frozen-thawed boar sperm
Estrada, Efrén (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste Oliveras, Marc (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2017
Resumen:	Adding cryopreservation media with reduced glutathione (GSH) has previously been shown to maintain the motility, membrane integrity and fertilizing ability of frozen-thawed boar sperm, although the effects of GSH on good (GFE) and poor freezability (PFE) ejaculates rely upon the intrinsic ejaculate freezability. The resilience to withstand freeze-thawing procedures has previously been related to the existence of a specific distribution of motile sperm subpopulations, which differs between GFE and PFE. Thus, the main aim of this study was to determine whether the addition of GSH to freezing media has any impact on the distribution of motile sperm subpopulations in GFE and PFE. With this purpose, 18 GFE and 13 PFE were cryopreserved with or without 2 mM GSH. Sperm quality and motile subpopulations were evaluated at 30 min and 4 h post-thawing. Three subpopulations were identified and the percentages of spermatozoa belonging to the fastest and most linear subpopulation, which was referred as 'SP1', decreased over post-thawing time. Good freezability ejaculates that were cryopreserved in the presence of 2 mM exhibited a significantly higher percentage of spermatozoa belonging to SP1 than the other combinations of treatment and freezability both at 30 min (mean ± SEM: GFE-C: 16. 6 ± 0. 4; GFE-GSH 27. 7 ± 0. 6) and 4 h post-thawing (GFE-C: 7. 8 ± 0. 2 vs.
Ayudas:	Ministerio de Economía y Competitividad AGL-2008-01792/GAN Ministerio de Economía y Competitividad JCI-2010-08620 Ministerio de Economía y Competitividad RYC-2014-15581
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Català
Documento:	Article ; recerca ; Versió publicada
Materia:	Porcs ; Espermatozoides ; Motilitat ; Esperma ; Anàlisi ; Criopreservació d'òrgans, teixits, etc. ; Motile sperm subpopulations ; Cryopreservation ; Reduced glutathione ; GSH
Publicado en:	Cryobiology, 2017 , ISSN 0011-2240

DOI: 10.1111/andr.12410
DOI: 10.1016/j.cryobiol.2017.07.002
PMID: 28697988

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