Brain Perihematoma Genomic Profile Following Spontaneous Human Intracerebral Hemorrhage
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vilalta, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
García-Berrocoso, Teresa (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Fernandez-Cadenas, Israel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Domingues-Montanari, Sophie (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Cuadrado Godia, Eloy (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Delgado Martínez, María Pilar (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ribo, Marc (Hospital Universitari Vall d'Hebron)
Martinez-Saez, Elena (Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques)
Ortega-Aznar, Arantxa (Hospital Universitari Vall d'Hebron)
Montaner, Joan (Hospital Universitari Vall d'Hebron)

Data:	2011
Resum:	Spontaneous intracerebral hemorrhage (ICH) represents about 15% of all strokes and is associated with high mortality rates. Our aim was to identify the gene expression changes and biological pathways altered in the brain following ICH. Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter. Affymetrix GeneChip platform for analysis of over 47,000 transcripts was conducted. Microarray Analysis Suite 5. 0 was used to process array images and the Ingenuity Pathway Analysis System was used to analyze biological mechanisms and functions of the genes. We identified 468 genes in the PH areas displaying a different expression pattern with a fold change between −3. 74 and +5. 16 when compared to the contralateral areas (291 overexpressed and 177 underexpressed). The top genes which appeared most significantly overexpressed in the PH areas codify for cytokines, chemokines, coagulation factors, cell growth and proliferation factors while the underexpressed codify for proteins involved in cell cycle or neurotrophins. Validation and replication studies at gene and protein level in brain samples confirmed microarray results. The genomic responses identified in this study provide valuable information about potential biomarkers and target molecules altered in the perihematomal regions.
Ajuts:	Instituto de Salud Carlos III CP09-00265 Instituto de Salud Carlos III CP09-00136 Instituto de Salud Carlos III FI09/00017 Instituto de Salud Carlos III PI05-322 Instituto de Salud Carlos III PI08-0361
Nota:	Altres ajuts: RENEVAS/RD06-0026-0010
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 6 (february 2011) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0016750
PMID: 21311749

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