Web of Science: 7 cites, Scopus: 7 cites, Google Scholar: cites
Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
Patiño, Tania (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Nogués, C (Carme) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Ibáñez, Elena (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Barrios, Leonardo (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data: 2012
Resum: Development of micro- and nanotechnology for the study of living cells, especially in the field of drug delivery, has gained interest in recent years. Although several studies have reported successful results in the internalization of micro- and nanoparticles in phagocytic cells, when nonphagocytic cells are used, the low internalization efficiency represents a limitation that needs to be overcome. It has been reported that covalent surface modification of micro- and nanoparticles increases their internalization rate. However, this surface modification represents an obstacle for their use as drug-delivery carriers. For this reason, the aim of the present study was to increase the capability for microparticle internalization of HeLa cells through the use of noncovalently bound transfection reagents: polyethyleneimine (PEI) Lipofectamine™ 2000 and FuGENE 6 ®. Both confocal microscopy and flow cytometry techniques allowed us to precisely quantify the efficiency of microparticle internalization by HeLa cells, yielding similar results. In addition, intracellular location of microparticles was analyzed through transmission electron microscopy and confocal microscopy procedures. Our results showed that free PEI at a concentration of 0. 05 mM significantly increased microparticle uptake by cells, with a low cytotoxic effect. As determined by transmission electron and confocal microscopy analyses, microparticles were engulfed by plasma-membrane projections during internalization, and 24 hours later they were trapped in a lysosomal compartment. These results show the potential use of noncovalently conjugated PEI in microparticle internalization assays.
Ajuts: Ministerio de Ciencia e Innovación TEC2008-06883-C03-03
Ministerio de Ciencia e Innovación TEC2011-29140-C03-03
Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-282
Agència de Gestió d'Ajuts Universitaris i de Recerca FI-DGR 2011
Agència de Gestió d'Ajuts Universitaris i de Recerca FI-DGR 2012
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: HeLa cells ; Drug delivery ; Endocytosis ; Internalization efficiency
Publicat a: International Journal of Nanomedicine, Vol. 7 (november 2012) , p. 5671-5682, ISSN 1178-2013

DOI: 10.2147/IJN.S34635
PMID: 23152683


12 p, 2.4 MB

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