Anal gas evacuation and colonic microbiota in patients with flatulence : effect of diet
Manichanh, Chaysavanh (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Eck, Anat (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Varela, Encarna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Roca, Joaquim (Institut de Biologia Molecular de Barcelona)
Clemente, José C.. (University of Colorado at Boulder, and Howard Hughes Medical Institute)
González, Antonio (University of Colorado at Boulder, and Howard Hughes Medical Institute)
Knights, Dan (University of Colorado at Boulder, and Howard Hughes Medical Institute)
Knight, Rob (University of Colorado at Boulder, and Howard Hughes Medical Institute)
Estrella, Sandra (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Hernandez, Carlos (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Guyonnet, Denis (Danone Research)
Accarino, Ana María (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Santos, Javier (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Malagelada Benaprés, Juan Ramón (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Guarner, Francisco (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Azpiroz Vidaur, Fernando (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Fecha:	2013
Resumen:	To characterise the influence of diet on abdominal symptoms, anal gas evacuation, intestinal gas distribution and colonic microbiota in patients complaining of flatulence. Patients complaining of flatulence (n=30) and healthy subjects (n=20) were instructed to follow their usual diet for 3 days (basal phase) and to consume a high-flatulogenic diet for another 3 days (challenge phase). During basal phase, patients recorded more abdominal symptoms than healthy subjects in daily questionnaires (5. 8±0. 3 vs 0. 4±0. 2 mean discomfort/pain score, respectively; p=<0. 0001) and more gas evacuations by an event marker (21. 9±2. 8 vs 7. 4±1. 0 daytime evacuations, respectively; p=0. 0001), without differences in the volume of gas evacuated after a standard meal (262±22 and 265±25 mL, respectively). On flatulogenic diet, both groups recorded more abdominal symptoms (7. 9±0. 3 and 2. 8±0. 4 discomfort/pain, respectively), number of gas evacuations (44. 4±5. 3 and 21. 7±2. 9 daytime evacuations, respectively) and had more gas production (656±52 and 673±78 mL, respectively; p<0. 05 vs basal diet for all). When challenged with flatulogenic diet, patients' microbiota developed instability in composition, exhibiting variations in the main phyla and reduction of microbial diversity, whereas healthy subjects' microbiota were stable. Taxa from Bacteroides fragilis or Bilophila wadsworthia correlated with number of gas evacuations or volume of gas evacuated, respectively. Patients complaining of flatulence have a poor tolerance of intestinal gas, which is associated with instability of the microbial ecosystem.
Ayudas:	Ministerio de Ciencia e Innovación FIS PI10-00902 Ministerio de Educación y Ciencia SAF 2009-07416
Nota:	Altres ajuts:This work was supported by the , Fundació La Marató TV3 (MARATV3_072010), the European Community's Seventh Framework Programme (FP7/2007-2013: IHMS, grant agreement HEALTH.2010.2.1.1-2) and a grant from Danone Research (France). Ciberehd is funded by the Instituto de Salud Carlos III.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Colonic Bacteria ; Colonic Fermentation ; Colonic Microflora ; Functional Bowel Disorder ; Visceral Sensitivity
Publicado en:	Gut, Vol. 63 (june 2013) , p. 401-408, ISSN 1468-3288

DOI: 10.1136/gutjnl-2012-303013
PMID: 23766444

8 p, 1.0 MB

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