A super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteria
Quiles Puchalt, Nuria (Instituto de Biomedicina de Valencia (IBV-CSIC))
Tormo Más, María Ángeles (Instituto Valenciano de Investigaciones Agrarias)
Campoy Sánchez, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Toledo Arana, Alejandro (Universidad Pública de Navarra. Instituto de Agrobiotecnología)
Monedero, Vicente (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Agroquimica y Tecnologia de Alimentos)
Lasa, Íñigo (Universidad Pública de Navarra. Instituto de Agrobiotecnología)
Novick, Richard P. (New York University Medical Center)
Christie, Gail E. (Virginia Commonwealth University School of Medicine. Department of Microbiology and Immunology)
Penadés, José R. (University of Glasgow. Institute of Infection, Immunity and Inflammation)

Data:	2013
Resum:	The propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. Here, we identified and characterized four different families of phage-encoded proteins that function as activators required for transcription of the late operons (morphogenetic and lysis genes) in a large group of phages infecting Gram-positive bacteria. These regulators constitute a super-family of proteins, here named late transcriptional regulators (Ltr), which share common structural, biochemical and functional characteristics and are unique to this group of phages. They are all small basic proteins, encoded by genes present at the end of the early gene cluster in their respective phage genomes and expressed under cI repressor control. To control expression of the late operon, the Ltr proteins bind to a DNA repeat region situated upstream of the ter S gene, activating its transcription. This involves the C-terminal part of the Ltr proteins, which control specificity for the DNA repeat region. Finally, we show that the Ltr proteins are the only phage-encoded proteins required for the activation of the packaging and lysis modules. In summary, we provide evidence that phage packaging and lysis is a conserved mechanism in Siphoviridae infecting a wide variety of Gram-positive bacteria.
Ajuts:	Ministerio de Ciencia e Innovación BIO2011-30503-C02-01 Ministerio de Ciencia e Innovación PIM2010EPA-00606 Ministerio de Ciencia e Innovación CSD2009-00006 Ministerio de Ciencia e Innovación DR08-0093
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Nucleic acids research, Vol. 41, issue 15 (Aug. 2013) , p. 7260-7275, ISSN 1362-4962

DOI: 10.1093/nar/gkt508
PMID: 23771138

16 p, 5.6 MB

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