Web of Science: 24 citations, Scopus: 20 citations, Google Scholar: citations,
Femtolitre chemistry assisted by microfluidic pen lithography
Carbonell Fernández, Carlos (Institut Catala de Nanociencia i Nanotecnologia)
Stylianou, Kyriakos C. (Institut Catala de Nanociencia i Nanotecnologia)
Hernando Campos, Jordi (Universitat Autònoma de Barcelona. Departament de Química)
Evangelio Castells, María Emilia (Institut Catala de Nanociencia i Nanotecnologia)
Barnett, Sarah A. (Harwell Science and Innovation Campus)
Nettikadan, Saju (Illinois Science and Technology Park)
Imaz, Inhar (Institut Catala de Nanociencia i Nanotecnologia)
Maspoch Comamala, Daniel (Institut Catala de Nanociencia i Nanotecnologia)

Date: 2013
Abstract: Chemical reactions at ultrasmall volumes are becoming increasingly necessary to study biological processes, to synthesize homogenous nanostructures and to perform high-throughput assays and combinatorial screening. Here we show that a femtolitre reaction can be realized on a surface by handling and mixing femtolitre volumes of reagents using a microfluidic stylus. This method, named microfluidic pen lithography, allows mixing reagents in isolated femtolitre droplets that can be used as reactors to conduct independent reactions and crystallization processes. This strategy overcomes the high-throughput limitations of vesicles and micelles and obviates the usually costly step of fabricating microdevices and wells. We anticipate that this process enables performing distinct reactions (acid-base, enzymatic recognition and metal-organic framework synthesis), creating multiplexed nanoscale metal-organic framework arrays, and screening combinatorial reactions to evaluate the crystallization of novel peptide-based materials. Chemical reactions on femtolitre scales are necessary to study confined biological processes. Here, the authors use a microfluidic pen lithography technique to perform a series of discrete femtoscale acid-base and synthetic reactions, and crystallizations on a surface with high registration accuracy.
Note: Número d'acord de subvenció EC/FP7/300390
Note: Número d'acord de subvenció MINECO/MAT2012-30994
Note: Número d'acord de subvenció MINECO/CTQ2011-16009-E
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials i que es distribueixin sota la mateixa llicència que regula l'obra original. Cal que es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Materials science ; Microfluidics ; Techniques and instrumentation
Published in: Nature communications, Vol. 4 (Jul. 2013) . art. 2173, ISSN 2041-1723

PMID: 23863998
DOI: 10.1038/ncomms3173

7 p, 1.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2018-01-27, last modified 2018-11-02

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