Meiotic abnormalities in metaphase I human spermatocytes from infertile males : frequencies, chromosomes involved, and relationship with polymorphic karyotype and seminal parameters
Sarrate Navas, Zaida (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Vidal, Francesca (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Blanco, Joan (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2014
Resum:	The aim of this study was to look in depth at the relationship between meiotic anomalies and male infertility, such as the determination of the chromosomes involved or the correlation with patient features. For this purpose, a total of 31 testicular tissue samples from individuals consulting for fertility problems were analyzed. Metaphase I cells were evaluated using a sequential methodology combining Leishman stained procedures and multiplex fluorescence in situ hybridization protocols. The number of chromosomal units and chiasmata count per bivalent were established and a hierarchical cluster analysis of the individuals was performed. The relationship of the seminogram and the karyotype over recombination were evaluated using Poisson regression models. Results obtained in this study show a significant percentage of infertile individuals with altered meiotic behavior, mostly specified as a reduction in chiasmata count in medium and large chromosomes, the presence of univalents, and the observation of tetraploid metaphases. Moreover, the number and the type of anomalies were found to be different between cells of the same individual, suggesting the coexistence of cell lines with normal meiotic behavior and cell lines with abnormalities. In addition, chromosomal abnormalities in metaphase I are significantly associated with oligozoospermia and/or polymorphic karyotype variants.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-00282 Ministerio de Ciencia e Innovación SAF2010-22241
Nota:	Altres ajuts: Universitat Autònoma de Barcelona CF-180034
Nota:	Resposta a un comentari sobre l'article: https://ddd.uab.cat/record/185096
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra, i la creació d'obres derivades, sempre que no sigui amb finalitats comercials i que es distribueixin sota la mateixa llicència que regula l'obra original. Cal que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Chiasmata count ; Human spermatocytes ; Multiplex fluorescence in situ hybridization ; Polymorphic karyotype ; Seminal parameters
Publicat a:	Asian Journal of Andrology, Vol. 16, Num 6 (July 2014) , p. 838-844, ISSN 1745-7262

DOI: 10.4103/1008-682X.135126
PMID: 25080930

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