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Non-synonymous genetic variation in exonic regions of canine Toll-like receptors
Cuscó, Anna (Vetgenomics)
Sánchez, Armand (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular)
Altet, Laura (Vetgenomics)
Ferrer, Lluís (Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University)
Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular)

Date: 2014
Abstract: Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) considered to be the primary sensors of pathogens in innate immunity. Genetic variants could be associated to differences in breed innate immune response to pathogens and thus to susceptibility to infections or autoimmune diseases. There is therefore great interest in the characterization of canine TLRs. Polymorphisms in canine TLRs have been characterized by massive sequencing after enrichment of their exonic regions. DNAs from 335 dogs (seven different breeds) and 100 wolves (two different populations) were used in pools. The ratio of SNP discovery was 76. 5% (in relation to CanFam 3. 1); 155 out of 204 variants identified were new. Functional annotation identified 64 non-synonymous variants (43 new), 73 synonymous variants (56 new) and 67 modifier variants (57 new). 12 out of 64 non-synonymous variants are breed or wolf specific. TLR5 has been found to be the most polymorphic among canine TLRs. Finally, a TaqMan OpenArray® plate containing 64 SNPs with a possible functional effect in the protein (4 frameshifts and 60 non-synonymous codons) has been designed and validated. Non-synonymous genetic variation has been characterized in exonic regions of canine Toll-like Receptors. The TaqMan OpenArray® plate developed to capture the individual variability that affects protein function will allow high-throughput genotyping either to study association to infection susceptibility or even TLR evolution in the canine genome. The online version of this article (doi:10. 1186/2052-6687-1-11) contains supplementary material, which is available to authorized users.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: TLRs ; Toll-like receptor ; Polymorphism ; SNPs ; Non-synonymous SNPs ; Canine ; Dog ; Innate immunity
Published in: Canine Genetics and Epidemiology, Vol. 1 (october 2014) , ISSN 2052-6687

PMID: 26401328
DOI: 10.1186/2052-6687-1-11

12 p, 520.6 KB

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Articles > Research articles
Articles > Published articles

 Record created 2018-01-29, last modified 2019-10-07

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