Evaluation of a new, rapid, simple test for the detection of influenza virus
Hurtado, Juan Carlos (Hospital Clínic i Provincial de Barcelona)
Mosquera, Maria del Mar (Hospital Clínic i Provincial de Barcelona)
de Lazzari, Elisa (Hospital Clínic i Provincial de Barcelona)
Martínez, Esteban (Hospital Clínic i Provincial de Barcelona)
Torner, Nuria (CIBER , Madrid)
Isanta, Ricard (Hospital Clínic i Provincial de Barcelona)
de Molina, Patricia (Hospital Clínic i Provincial de Barcelona)
Pumarola Suñé, Tomàs (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Marcos, María Ángeles (Hospital Clínic i Provincial de Barcelona)
Vila Estapé, Jordi (Hospital Clínic i Provincial de Barcelona)
Universitat Autònoma de Barcelona

Data:	2015
Resum:	Influenza virus infections are responsible for significant morbidity and mortality in both pediatric and adult populations worldwide. Rapid and accurate diagnosis of influenza is necessary for appropriate patient management during the influenza season and for optimal utilization of anti-influenza therapy. We prospectively tested the accuracy of a simple and rapid diagnostic method. Ninety-eight samples (nasal and pharyngeal swabs) from patients with upper respiratory tract infection symptoms who presented to primary healthcare centres in Barcelona (Spain) were prospectively analyzed. The samples were collected as part of influenza surveillance program. Samples that had enough volume to make the new test after aliquoting the amount needed to perform routine tests were included. None of the samples were pre-selected as a result of their status in relation to influenza virus. Samples were analyzed by in-house real-time PCR and Alere™ i Influenza A & B (Alere™ i), which uses isothermal amplification of nucleic acids for the qualitative detection of influenza A and B in nasal swabs transported in viral transport media. The two techniques were compared by positive percent agreement (PPA) and negative percent agreement (NPA). Statistical analysis was performed with Stata. Of the 98 samples analysed 90 were concordant; 46 (46. 9%) were positive and 44 (44. 9%) were negative. Five samples showed invalid results with the Alere™ i test and could be not re-tested due to insufficient sample volume and were not included in the final statistical analysis. In the 93 remaining samples, the Alere™ i test showed 97% of accuracy having correctly classified 90 samples. We obtained discordant results in 3 samples (3%). The PPA was 93. 8% for influenza A and 94. 1% for influenza B, and NPA was 100% for influenza A and influenza B virus. In addition, the Alere™ i was very rapid (15 minutes or less) and extremely easy to use. The Alere™ i test provided a good correlation compared to the real-time PCR test for the diagnosis of influenza. Since this method can be performed in minutes, it allows immediate, accurate clinical decisions to prescribe appropriate antiviral treatment or isolation of patients.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Influenza Virus A and B ; Isothermal amplification ; Real time PCR ; Rapid test
Publicat a:	BMC Infectious diseases, Vol. 15 (february 2015) , ISSN 1471-2334

Erratum: https://ddd.uab.cat/record/254450
DOI: 10.1186/s12879-015-0775-5
PMID: 25656393

4 p, 672.8 KB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats