An experimental study of muscular injury repair in a mouse model of notexin-induced lesion with EPI® technique
Abat, Ferran (ReSport Clinic, Barcelona)
Valles, Soraya L. (Universitat de València. Departament de Fisiologia)
Gelber Ghertner, Pablo Eduardo (Institut d'Investigació Biomèdica Sant Pau)
Polidori, Fernando (Cerede Sports Medicine (Barcelona). Department of Sports Rehabilitation)
Jorda, Adrian (Universitat de València. Departament de Fisiologia)
García Herreros, Sergio (Universitat de València. Departament de Fisiologia)
Monllau García, Joan Carles (Institut Hospital del Mar d'Investigacions Mèdiques)
Sanchez Ibáñez, José Manuel (Cerede Sports Medicine (Barcelona). Department of Sports Rehabilitation)
Universitat Autònoma de Barcelona

Fecha:	2015
Resumen:	The mechanisms of muscle injury repair after EPI® technique, a treatment based on electrical stimulation, have not been described. This study determines whether EPI® therapy could improve muscle damage. Twenty-four rats were divided into a control group, Notexin group (7 and 14 days) and a Notexin + EPI group. To induce muscle injury, Notexin was injected in the quadriceps of the left extremity of rats. Pro-inflammatory interleukin 1-beta (IL-1beta) and tumoral necrosis factor-alpha (TNF-alpha) were determined by ELISA. The expression of receptor peroxisome gamma proliferator activator (PPAR-gamma), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-1 (VEGF-R1) were determined by western-blot. The plasma levels of TNF-alpha and IL-1beta in Notexin-injured rats showed a significant increase compared with the control group. EPI® produced a return of TNF-alpha and IL-1beta values to control levels. PPAR-gamma expression diminished injured quadriceps muscle in rats. EPI® increased PPAR-gamma, VEGF and VEGF-R1 expressions. EPI® decreased plasma levels of pro-inflammatory TNF-alpha and IL-1beta and increased anti-inflammatory PPAR-gamma and proangiogenic factors as well as VEGF and VEGF-R1 expressions. The EPI® technique may affect inflammatory mediators in damaged muscle tissue and influences the new vascularization of the injured area. These results suggest that EPI® might represent a useful new therapy for the treatment of muscle injuries. Although our study in rats may represent a valid approach to evaluate EPI® treatment, studies designed to determine how the EPI® treatment may affect recovery of injury in humans are needed.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	EPI ; Technique ; Notexin-induced ; Muscle ; Injury
Publicado en:	BMC Sports Science, Medicine and Rehabilitation, Vol. 7 (april 2015) , ISSN 2052-1847

DOI: 10.1186/s13102-015-0002-0
PMID: 25897404

7 p, 1006.5 KB

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