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Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)
Montero, Daniel (Universidad de Las Palmas de Gran Canaria)
Terova, Genciana (Università degli Studi dell'Insubria. Dipartimento di Biotecnologie e Scienze della Vita)
Rimoldi, Simona (Università degli Studi dell'Insubria. Dipartimento di Biotecnologie e Scienze della Vita)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Negrin, Davinia (Universidad de Las Palmas de Gran Canaria)
Zamorano, María Jesús (Universidad de Las Palmas de Gran Canaria)
Izquierdo, Marisol (Universidad de Las Palmas de Gran Canaria (ULPGC))

Date: 2015
Abstract: Dietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or α-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c - fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c - fos after 20 min. ARA increased expression of the 11β-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.
Note: Número d'acord de subvenció EC/FP7/288925
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Stress-related gene expression ; Fatty acids ; Adrenocorticotrophin hormone-induced stress response ; Nutritional modulation of steroidogenesis ; Dicentrarchus labrax ; ACTH, adrenocorticotrophin hormone ; ALA, α-linolenic acid ; ARA, arachidonic acid ; COX, cyclo-oxygenase ; CYP11b, cytochrome P450 11β ; GR, glucocorticoid receptor ; HSP, heat shock protein ; LA, linoleic acid ; LOX, lipo-oxygenase ; PKA, protein kinase A ; PLA2, phospholipase A2 ; StAR, steroidogenic acute regulatory protein
Published in: Journal of nutritional science, Vol. 4 (May 2015) , e16, ISSN 2048-6790

DOI: 10.1017/jns.2015.6
PMID: 26090096

13 p, 2.1 MB

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Articles > Research articles
Articles > Published articles

 Record created 2018-01-31, last modified 2020-08-09

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