Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
Garcia, Carolina (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Delprat Obeaga, Alejandra (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ruiz, Alfredo (Ruiz Panadero) (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Valente, Vera L. S. (Universidade Federal do Rio Grande do Sul. Departamento de Genética, Instituto de Biociências)

Data:	2015
Resum:	Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromosomes based on a handful of genetic and physical markers. This paucity of markers was particularly striking in the metacentric chromosome II, comprised two similarly sized arms, IIL and IIR, traditionally considered homologous to Muller elements C and B, respectively. In this paper we present the cytological mapping of 22 new gene markers to increase the number of markers mapped by in situ hybridization and to test the assignment of scaffolds to the polytene chromosome II arms. For this purpose, we generated, by polymerase chain reaction amplification, one or two gene probes from each scaffold assigned to the chromosome II arms and mapped these probes to the Gd-H4-1 strain's polytene chromosomes by nonfluorescent in situ hybridization. Our findings show that chromosome arms IIL and IIR correspond to Muller elements B and C, respectively, directly contrasting the current homology assignments in D. willistoni and constituting a major reassignment of the scaffolds to chromosome II arms.
Ajuts:	Ministerio de Ciencia e Innovación BFU2011-30476
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Drosophila willistoni ; Polytene chromosome II ; Physical markers ; In situ hybridization ; Muller elements
Publicat a:	G3, Vol. 5, issue 12 (october 2015) , p. 2559-2566, ISSN 2160-1836

DOI: 10.1534/g3.115.021311
PMID: 26438290

8 p, 3.0 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats